| 1st | Abigail Kochevar | Rock Canyon (Highlands Ranch, CO) | Final | | 00:24.45 |
| 2nd | Kaylee Gassen | Chaparral (Parker, CO) | Final | | 00:24.92 |
| 3rd | Alex Reddington | St. Mary's Academy (Englewood, CO) | Final | | 00:25.13 |
| 4th | Sofia Zinis | Mullen (Denver, CO) | Final | | 00:25.46 |
| 5th | Parker Henry | Mountain Vista (Highlands Ranch, CO) | Final | | 00:25.47 |
| 6th | Molly Nankey | Grandview (Aurora, CO) | Final | | 00:25.68 |
| 7th | Ellie Furuta | Grandview (Aurora, CO) | Final | | 00:25.91 |
| 8th | Veronica Postolski | Rock Canyon (Highlands Ranch, CO) | Final | | 00:25.99 |
| 9th | Courtney Linton | Legend (Parker, CO) | Final | | 00:25.99 |
| 10th | Anne Younger | Colorado Academy (Denver, CO) | Final | | 00:26.03 |
| 11th | Madison Coonts | Rock Canyon (Highlands Ranch, CO) | Final | | 00:26.05 |
| 12th | Aly Gallagher | Colorado Academy (Denver, CO) | Final | | 00:26.08 |
| 13th | Becca Kholos | Legend (Parker, CO) | Final | | 00:26.29 |
| 14th | Chiara Robinson | Rock Canyon (Highlands Ranch, CO) | Final | | 00:26.33 |
| 15th | Kristen Torres | Chaparral (Parker, CO) | Final | | 00:26.40 |
| 16th | Michaela Sandeno | Chaparral (Parker, CO) | Final | | 00:26.58 |
| 17th | Abigail Smith | Grandview (Aurora, CO) | Final | | 00:26.68 |
| 18th | Naciye Cheang | Grandview (Aurora, CO) | Final | | 00:26.76 |
| 19th | Claire Neid | George Washington (Denver, CO) | Final | | 00:26.79 |
| 20th | Tori Lim | Colorado Academy (Denver, CO) | Final | | 00:27.06 |
| 21st | Isa Faddick | St. Mary's Academy (Englewood, CO) | Final | | 00:27.26 |
| 22nd | Jackie Melberg | Colorado Academy (Denver, CO) | Final | | 00:27.41 |
| 23rd | Grace Hicks | Mullen (Denver, CO) | Final | | 00:27.62 |
| 24th | Lexi James | Centaurus (Lafayette, CO) | Final | | 00:27.63 |
| 25th | Sarah Macdougall | Doherty (Colorado Springs, CO) | Final | | 00:27.64 |
| 26th | Julianna Krinsky | Pueblo County (Pueblo, CO) | Final | | 00:27.80 |
| 27th | Elena Staver | George Washington (Denver, CO) | Final | | 00:27.84 |
| 28th | Lexi Killinger | George Washington (Denver, CO) | Final | | 00:27.90 |
| 29th | Ellianna Brown | Legend (Parker, CO) | Final | | 00:28.28 |
| 30th | Marlayna McClay | Mountain Vista (Highlands Ranch, CO) | Final | | 00:28.34 |
| 31st | Sarah Boyer | Centaurus (Lafayette, CO) | Final | | 00:28.40 |
| 32nd | Lauren Brown | Mountain Vista (Highlands Ranch, CO) | Final | | 00:28.60 |
| 33rd | Sarah Wiener | George Washington (Denver, CO) | Final | | 00:28.72 |
| 34th | Luna Boydstun | Doherty (Colorado Springs, CO) | Final | | 00:28.78 |
| 35th | Isha Banerjee | Centaurus (Lafayette, CO) | Final | | 00:29.04 |
| 36th | Natalie Stammel | Chaparral (Parker, CO) | Final | | 00:29.10 |
| 37th | Ashlyn Bostic | Centaurus (Lafayette, CO) | Final | | 00:29.34 |
| 38th | Dana Nguyen | ThunderRidge (Highlands Ranch, CO) | Final | | 00:29.42 |
| 39th | Sara Arnold | Mountain Vista (Highlands Ranch, CO) | Final | | 00:29.52 |
| 40th | Emily Milla | Legend (Parker, CO) | Final | | 00:29.59 |
| 41st | Abbey Lau | Summit (Frisco, CO) | Final | | 00:29.64 |
| 42nd | Anna Scheitler | Mullen (Denver, CO) | Final | | 00:29.80 |
| 43rd | Emily Schauer | Doherty (Colorado Springs, CO) | Final | | 00:30.11 |
| 44th | Emma Cowie | Mountain Vista (Highlands Ranch, CO) | Final | | 00:30.15 |
| 45th | Kiersten Johnson | Mountain Vista (Highlands Ranch, CO) | Final | | 00:30.42 |
| 46th | Delaney McLaughlin | Doherty (Colorado Springs, CO) | Final | | 00:30.56 |
| 47th | Lea Haertwig | Mountain Vista (Highlands Ranch, CO) | Final | | 00:30.57 |
| 48th | Chloe Callanan | Mullen (Denver, CO) | Final | | 00:30.69 |
| 49th | Megan Callister | Mountain Vista (Highlands Ranch, CO) | Final | | 00:30.79 |
| 50th | Kate Vaillant | Mountain Vista (Highlands Ranch, CO) | Final | | 00:31.13 |
| 51st | Elena Trujillo | Pueblo County (Pueblo, CO) | Final | | 00:31.31 |
| 52nd | Morgan MacLean | Mountain Vista (Highlands Ranch, CO) | Final | | 00:31.86 |
| 53rd | Shiyue Zhang | Mountain Vista (Highlands Ranch, CO) | Final | | 00:32.00 |
| 54th | Katia Medina | ThunderRidge (Highlands Ranch, CO) | Final | | 00:32.06 |
| 55th | Abby Wickoren | Mountain Vista (Highlands Ranch, CO) | Final | | 00:32.70 |
| 56th | Anissa Emanouzi | Castle View/Douglas County (Castle Rock, CO) | Final | | 00:32.96 |
| 57th | Jacqueline Laing | Mountain Vista (Highlands Ranch, CO) | Final | | 00:33.05 |
| 58th | Tyler Kiefer | ThunderRidge (Highlands Ranch, CO) | Final | | 00:33.09 |
| 59th | Chloe Blackhurst | Castle View/Douglas County (Castle Rock, CO) | Final | | 00:33.48 |
| 60th | Savannah Raisor | Mountain Vista (Highlands Ranch, CO) | Final | | 00:33.55 |
| 61st | Maddy Worley | Mountain Vista (Highlands Ranch, CO) | Final | | 00:34.25 |
| 62nd | ShaneaRae Cordova | Pueblo County (Pueblo, CO) | Final | | 00:34.32 |
| 63rd | Elissa Wardell | ThunderRidge (Highlands Ranch, CO) | Final | | 00:34.68 |
| 64th | Brey Murray | Mountain Vista (Highlands Ranch, CO) | Final | | 00:34.70 |
| 65th | Jacklyn Cole | Mountain Vista (Highlands Ranch, CO) | Final | | 00:35.40 |
| 66th | Frances Forward | St. Mary's Academy (Englewood, CO) | Final | | 00:35.60 |
| 67th | Taylor Holst | Mountain Vista (Highlands Ranch, CO) | Final | | 00:35.73 |
| 68th | Chloe Schmidt | Castle View/Douglas County (Castle Rock, CO) | Final | | 00:36.57 |
| 69th | Cassidy Lawson | Summit (Frisco, CO) | Final | | 00:37.55 |
| 70th | Makayla Jones | Centaurus (Lafayette, CO) | Final | | 00:37.97 |
| 71st | Raven Aiken | Mountain Vista (Highlands Ranch, CO) | Final | | 00:38.30 |
| 72nd | Isabella Oldenburg | Castle View/Douglas County (Castle Rock, CO) | Final | | 00:39.38 |
| 73rd | Cassidy Raisor | Mountain Vista (Highlands Ranch, CO) | Final | | 00:39.41 |
| 74th | Gina Escobedo | Denver North (Denver, CO) | Final | | 00:42.22 |
| 75th | Samantha Mejia | Denver North (Denver, CO) | Final | | 00:44.93 |
| 76th | Abigail Garcia | Denver North (Denver, CO) | Final | | 00:46.25 |
| 77th | Briana Ramirez | Mitchell (Colorado Springs, CO) | Final | | 00:49.01 |
| 78th | Shukri Hassan | Denver North (Denver, CO) | Final | | 00:57.01 |
