| 1st | Abbey Craig | Omaha Central (Omaha, NE) | Final | | 00:26.92 |
| 2nd | Jacque McCullough | Bellevue East (Bellevue, NE) | Final | | 00:27.78 |
| 3rd | Ann Fox | Omaha North (Omaha, NE) | Final | | 00:28.09 |
| 4th | Kylie Westphal | Omaha Central (Omaha, NE) | Final | | 00:28.37 |
| 5th | Frances Gray | Omaha Central (Omaha, NE) | Final | | 00:28.94 |
| 6th | Daniella Hernandez | Omaha Central (Omaha, NE) | Final | | 00:29.00 |
| 7th | Emma Nieland | Omaha Central (Omaha, NE) | Final | | 00:29.07 |
| 8th | Mia Spencer | Omaha Central (Omaha, NE) | Final | | 00:29.47 |
| 9th | Lauren Anderson | Omaha Central (Omaha, NE) | Final | | 00:29.57 |
| 10th | Shay Macafee | Omaha Central (Omaha, NE) | Final | | 00:29.66 |
| 11th | Bianca Martinez | Omaha Central (Omaha, NE) | Final | | 00:30.50 |
| 12th | Isabel Kolb | Omaha North (Omaha, NE) | Final | | 00:31.04 |
| 13th | Chloe Jones | Omaha Central (Omaha, NE) | Final | | 00:31.07 |
| 14th | Madeline DeMare | Omaha Central (Omaha, NE) | Final | | 00:31.11 |
| 15th | Alison Doody | Omaha Central (Omaha, NE) | Final | | 00:31.21 |
| 16th | Elena Conyers-Gaines | Omaha North (Omaha, NE) | Final | | 00:31.54 |
| 17th | Kallie Baker | Omaha Central (Omaha, NE) | Final | | 00:31.65 |
| 18th | Kassie Ronei | Bryan (Omaha, NE) | Final | | 00:31.65 |
| 19th | Larisa Hurtado | Omaha Central (Omaha, NE) | Final | | 00:31.84 |
| 20th | Laila Klare | Omaha Central (Omaha, NE) | Final | | 00:31.97 |
| 21st | Devlyn Pithan | Omaha Central (Omaha, NE) | Final | | 00:32.29 |
| 22nd | Ashley Stehlik | Bellevue East (Bellevue, NE) | Final | | 00:32.36 |
| 23rd | Maecyn Sieborg | Omaha North (Omaha, NE) | Final | | 00:32.56 |
| 24th | Isabella Shradar | Omaha Central (Omaha, NE) | Final | | 00:32.65 |
| 25th | Emily Quackenbush | Omaha North (Omaha, NE) | Final | | 00:32.70 |
| 26th | Ruby Nelson | Bryan (Omaha, NE) | Final | | 00:32.75 |
| 27th | Madison Langle | Bryan (Omaha, NE) | Final | | 00:33.06 |
| 28th | Emily Engel | Omaha Central (Omaha, NE) | Final | | 00:33.30 |
| 29th | Katheryn Cunningham | Bellevue East (Bellevue, NE) | Final | | 00:33.40 |
| 30th | Olivia Theophilus | Omaha Central (Omaha, NE) | Final | | 00:33.62 |
| 31st | Riley Sanden | Omaha Central (Omaha, NE) | Final | | 00:33.63 |
| 32nd | Macrina Barber-Holmes | Omaha North (Omaha, NE) | Final | | 00:33.63 |
| 33rd | Sophia Sidzyik | Omaha Central (Omaha, NE) | Final | | 00:33.68 |
| 34th | Tali Shradar | Omaha Central (Omaha, NE) | Final | | 00:33.72 |
| 35th | Valkyrie Bostwick | Benson (Omaha, NE) | Final | | 00:33.76 |
| 36th | Keneccia Garzon | Omaha South (Omaha, NE) | Final | | 00:33.76 |
| 37th | Elizabeth Mendoza | Omaha South (Omaha, NE) | Final | | 00:33.97 |
| 38th | Mikayla Wingate | Bellevue East (Bellevue, NE) | Final | | 00:34.07 |
| 39th | Maddie Brewer | Bryan (Omaha, NE) | Final | | 00:34.28 |
| 40th | Victoria Schradar | Omaha Central (Omaha, NE) | Final | | 00:34.36 |
| 41st | Ava Marx | Bellevue East (Bellevue, NE) | Final | | 00:34.36 |
| 42nd | Arianna Mariucci | Omaha North (Omaha, NE) | Final | | 00:34.54 |
| 43rd | Britney Guzman | Omaha South (Omaha, NE) | Final | | 00:34.54 |
| 44th | Pangaea Kaan | Omaha Central (Omaha, NE) | Final | | 00:34.69 |
| 45th | Jessica Darden | Benson (Omaha, NE) | Final | | 00:34.82 |
| 46th | Sami Stuart | Omaha North (Omaha, NE) | Final | | 00:34.99 |
| 47th | Annabelle Biersdorfer | Bellevue East (Bellevue, NE) | Final | | 00:35.33 |
| 48th | Cerenity Galante | Benson (Omaha, NE) | Final | | 00:35.52 |
| 49th | Angelina Zamora | Omaha South (Omaha, NE) | Final | | 00:35.64 |
| 50th | Ava Sherman | Omaha Central (Omaha, NE) | Final | | 00:35.68 |
| 51st | Moriah Liebenthal | Bellevue East (Bellevue, NE) | Final | | 00:36.26 |
| 52nd | Katie Schuman | Omaha Central (Omaha, NE) | Final | | 00:36.69 |
| 53rd | Emily Beckham | Bellevue East (Bellevue, NE) | Final | | 00:36.94 |
| 54th | Itzel Correa | Omaha South (Omaha, NE) | Final | | 00:36.98 |
| 55th | Emily Abrego-Zurceno | Bryan (Omaha, NE) | Final | | 00:37.30 |
| 56th | Leah Trumble | Bellevue East (Bellevue, NE) | Final | | 00:37.31 |
| 57th | Elise Lutz | Bellevue East (Bellevue, NE) | Final | | 00:37.36 |
| 58th | Summer Metz | Bellevue East (Bellevue, NE) | Final | | 00:37.67 |
| 59th | Alyssa Sturtevant | Omaha South (Omaha, NE) | Final | | 00:37.89 |
| 60th | Allison Draper | Bellevue East (Bellevue, NE) | Final | | 00:37.97 |
| 61st | Haylee Johnson | Bellevue East (Bellevue, NE) | Final | | 00:38.01 |
| 62nd | Katheryn Alvarez | Bryan (Omaha, NE) | Final | | 00:38.20 |
| 63rd | Lakyn Richard | Benson (Omaha, NE) | Final | | 00:38.32 |
| 64th | Alaina Grisafe | Bellevue East (Bellevue, NE) | Final | | 00:38.33 |
| 65th | Sonya Raymundo | Bryan (Omaha, NE) | Final | | 00:38.55 |
| 66th | Kyra Piper | Bellevue East (Bellevue, NE) | Final | | 00:39.02 |
| 67th | Victoria Ajayi | Omaha South (Omaha, NE) | Final | | 00:39.55 |
| 68th | Maiah Tomsu | Bellevue East (Bellevue, NE) | Final | | 00:39.89 |
| 69th | Victoria Hill | Bryan (Omaha, NE) | Final | | 00:40.39 |
| 70th | Savannah Pearrow | Bellevue East (Bellevue, NE) | Final | | 00:40.46 |
| 71st | Emilie Peterson | Bellevue East (Bellevue, NE) | Final | | 00:40.55 |
| 72nd | Abigail Sanchez | Bryan (Omaha, NE) | Final | | 00:41.22 |
| 73rd | Fatima Leiva | Bryan (Omaha, NE) | Final | | 00:41.42 |
| 74th | Melissa Merida-Cardone | Omaha South (Omaha, NE) | Final | | 00:41.52 |
| 75th | Jayla Incontro | Omaha Central (Omaha, NE) | Final | | 00:41.70 |
| 76th | Mackenzie Sullivan | Bryan (Omaha, NE) | Final | | 00:42.64 |
| 77th | Auroa Taylor | Bryan (Omaha, NE) | Final | | 00:43.50 |
| 78th | Anna Haubensak | Bellevue East (Bellevue, NE) | Final | | 00:44.57 |
| 79th | Lexi Hanson | Bellevue East (Bellevue, NE) | Final | | 00:45.00 |
| 80th | Haley Wienk | Bellevue East (Bellevue, NE) | Final | | 00:53.36 |
