| 1st | Sydney Melnick | George Washington (Denver, CO) | Final | | 00:26.28 |
| 2nd | Isa Faddick | St. Mary's Academy (Englewood, CO) | Final | | 00:27.27 |
| 3rd | Claire Neid | George Washington (Denver, CO) | Final | | 00:27.42 |
| 4th | Lexi Killinger | George Washington (Denver, CO) | Final | | 00:27.76 |
| 5th | Annika Moen | George Washington (Denver, CO) | Final | | 00:27.97 |
| 6th | Nina Crouch | Denver East (Denver, CO) | Final | | 00:28.21 |
| 7th | Mia Nelson | Denver East (Denver, CO) | Final | | 00:28.52 |
| 8th | Mia Keller | George Washington (Denver, CO) | Final | | 00:29.35 |
| 9th | Ava Baumann | Far Northeast Warriors (Denver, CO) | Final | | 00:29.36 |
| 10th | Hazel Creighton | Far Northeast Warriors (Denver, CO) | Final | | 00:29.46 |
| 11th | Jordan Garelick | Denver East (Denver, CO) | Final | | 00:30.00 |
| 12th | Ingrid Backes | Denver East (Denver, CO) | Final | | 00:30.14 |
| 13th | Gabby Camp-Lagueux | George Washington (Denver, CO) | Final | | 00:30.51 |
| 14th | Kathryn Beegle | Denver South (Denver, CO) | Final | | 00:30.52 |
| 15th | Elizabeth Torgerson | George Washington (Denver, CO) | Final | | 00:30.63 |
| 16th | Rachell Brandorff | Thomas Jefferson (Denver, CO) | Final | | 00:30.75 |
| 17th | Samantha Rivera | Denver East (Denver, CO) | Final | | 00:30.86 |
| 18th | Julia Turnbow | Thomas Jefferson (Denver, CO) | Final | | 00:30.94 |
| 19th | Abi Bosworth | Denver West (Denver, CO) | Final | | 00:31.03 |
| 20th | Natalie Shelden | George Washington (Denver, CO) | Final | | 00:31.32 |
| 21st | Catherine Bakken | George Washington (Denver, CO) | Final | | 00:31.67 |
| 22nd | Olivia Fullerton | Thomas Jefferson (Denver, CO) | Final | | 00:31.78 |
| 23rd | Emily Shepardson | St. Mary's Academy (Englewood, CO) | Final | | 00:31.87 |
| 24th | Margaret Torgerson | Denver East (Denver, CO) | Final | | 00:31.94 |
| 25th | Gigi Emerson | George Washington (Denver, CO) | Final | | 00:32.17 |
| 26th | Emma Herman | George Washington (Denver, CO) | Final | | 00:32.36 |
| 27th | Maria Ricalday | Lincoln (Denver, CO) | Final | | 00:32.41 |
| 28th | Mirsey Crowley | Denver West (Denver, CO) | Final | | 00:32.50 |
| 29th | Jacqueline Danahey | Denver East (Denver, CO) | Final | | 00:32.66 |
| 30th | Nora Drewno | Denver North (Denver, CO) | Final | | 00:33.10 |
| 31st | Amanda Keeler | Lincoln (Denver, CO) | Final | | 00:33.26 |
| 32nd | Camille Merrick | Denver South (Denver, CO) | Final | | 00:33.57 |
| 33rd | Pippi Thompson | Denver South (Denver, CO) | Final | | 00:33.66 |
| 34th | Emily Main | Lincoln (Denver, CO) | Final | | 00:33.93 |
| 35th | Maude Pointet | George Washington (Denver, CO) | Final | | 00:34.12 |
| 36th | Judith Dietz | Lincoln (Denver, CO) | Final | | 00:34.34 |
| 37th | Anna Manning | Denver South (Denver, CO) | Final | | 00:34.67 |
| 38th | Isabel Forward | St. Mary's Academy (Englewood, CO) | Final | | 00:34.84 |
| 39th | Kennedy Perry | Thomas Jefferson (Denver, CO) | Final | | 00:34.88 |
| 40th | Chloe Applegate | Denver South (Denver, CO) | Final | | 00:35.06 |
| 41st | Deanna Main | Lincoln (Denver, CO) | Final | | 00:35.14 |
| 42nd | Chava Baldiva | Denver South (Denver, CO) | Final | | 00:35.17 |
| 43rd | Tate Lacasse | Denver South (Denver, CO) | Final | | 00:35.58 |
| 44th | Kaelyne Gomez | Denver East (Denver, CO) | Final | | 00:35.71 |
| 45th | Callie McKenna | St. Mary's Academy (Englewood, CO) | Final | | 00:35.77 |
| 46th | Frances Forward | St. Mary's Academy (Englewood, CO) | Final | | 00:35.83 |
| 47th | Hannah Pogue | St. Mary's Academy (Englewood, CO) | Final | | 00:36.24 |
| 48th | Violet Virnich | Denver West (Denver, CO) | Final | | 00:36.42 |
| 49th | Gabriela Duran | Denver West (Denver, CO) | Final | | 00:36.52 |
| 50th | Anna Howard | George Washington (Denver, CO) | Final | | 00:36.56 |
| 51st | Nina Brinkman | St. Mary's Academy (Englewood, CO) | Final | | 00:36.58 |
| 52nd | Bridget Binder | St. Mary's Academy (Englewood, CO) | Final | | 00:36.58 |
| 53rd | Melina Kilen | Denver North (Denver, CO) | Final | | 00:36.62 |
| 54th | Kassidy Zube | Denver South (Denver, CO) | Final | | 00:36.77 |
| 55th | Ashley McCord | Lincoln (Denver, CO) | Final | | 00:36.84 |
| 56th | Janette Chavez | Far Northeast Warriors (Denver, CO) | Final | | 00:36.85 |
| 57th | Halle Bristow | Thomas Jefferson (Denver, CO) | Final | | 00:37.29 |
| 58th | Miranda Garo | Far Northeast Warriors (Denver, CO) | Final | | 00:37.34 |
| 59th | Anna Heimel | George Washington (Denver, CO) | Final | | 00:38.06 |
| 60th | Kassandra Escoto | Denver North (Denver, CO) | Final | | 00:38.24 |
| 61st | Gina Kinley | Denver West (Denver, CO) | Final | | 00:38.41 |
| 62nd | Edith Almanzan-Prieto | Lincoln (Denver, CO) | Final | | 00:38.59 |
| 63rd | Camille Moore | Thomas Jefferson (Denver, CO) | Final | | 00:38.92 |
| 64th | Kathy Nguyen | Lincoln (Denver, CO) | Final | | 00:39.04 |
| 65th | Mariana Ledezma | Lincoln (Denver, CO) | Final | | 00:39.05 |
| 66th | Phoebe Fanganello | Denver East (Denver, CO) | Final | | 00:39.33 |
| 67th | Audrey Wilhite | Lincoln (Denver, CO) | Final | | 00:39.84 |
| 68th | Audrey State | St. Mary's Academy (Englewood, CO) | Final | | 00:39.85 |
| 69th | Alina Gallegos | George Washington (Denver, CO) | Final | | 00:41.20 |
| 70th | Lameine VanDerMeij | Denver East (Denver, CO) | Final | | 00:41.22 |
| 71st | Gabriela Lopez | Lincoln (Denver, CO) | Final | | 00:41.38 |
| 72nd | Jazlyn Rodriguez | Denver North (Denver, CO) | Final | | 00:41.53 |
| 73rd | Matty Wacker | Far Northeast Warriors (Denver, CO) | Final | | 00:42.30 |
| 74th | Kristy Romero | Lincoln (Denver, CO) | Final | | 00:42.41 |
| 75th | Courtney Davis | Denver South (Denver, CO) | Final | | 00:42.84 |
| 76th | Bella Durden | Denver North (Denver, CO) | Final | | 00:43.06 |
| 77th | Marisa Lopez | Denver West (Denver, CO) | Final | | 00:43.20 |
| 78th | Kate Windler | St. Mary's Academy (Englewood, CO) | Final | | 00:43.39 |
| 79th | Carlene Beaton | Denver North (Denver, CO) | Final | | 00:43.61 |
| 80th | Gina Escobedo | Denver North (Denver, CO) | Final | | 00:43.92 |
| 81st | Gnaleh Kande | Far Northeast Warriors (Denver, CO) | Final | | 00:43.94 |
| 82nd | Asiane Jackson | Thomas Jefferson (Denver, CO) | Final | | 00:45.05 |
| 83rd | Felicia Sanchez | Denver North (Denver, CO) | Final | | 00:45.29 |
| 84th | Isabelle Ryan | Thomas Jefferson (Denver, CO) | Final | | 00:45.67 |
| 85th | Adriana Gallegos | Lincoln (Denver, CO) | Final | | 00:46.13 |
| 86th | Valyntine Lonergan | Thomas Jefferson (Denver, CO) | Final | | 00:46.36 |
| 87th | Alondra Arreola | Far Northeast Warriors (Denver, CO) | Final | | 00:46.48 |
| 88th | Renee Aburto | Thomas Jefferson (Denver, CO) | Final | | 00:46.98 |
| 89th | Nancy Rodriguez | Thomas Jefferson (Denver, CO) | Final | | 00:47.35 |
| 90th | Trinity Pappas | Denver West (Denver, CO) | Final | | 00:47.80 |
| 91st | Jennifer Recinos | Lincoln (Denver, CO) | Final | | 00:48.03 |
| 92nd | Andrea Orozco | Lincoln (Denver, CO) | Final | | 00:48.14 |
| 93rd | Jammy Johnson | Denver West (Denver, CO) | Final | | 00:49.15 |
| 94th | Abigail Garcia | Denver North (Denver, CO) | Final | | 00:49.84 |
| 95th | Paula Ruiz | Lincoln (Denver, CO) | Final | | 00:51.58 |
| 96th | Jacsiri Bautista | Denver South (Denver, CO) | Final | | 00:52.67 |
| 97th | Getssemany Rivera | Lincoln (Denver, CO) | Final | | 00:52.69 |
| 98th | Lindsey Connolly | Denver West (Denver, CO) | Final | | 00:52.75 |
| 99th | Alexis Tejada | Denver South (Denver, CO) | Final | | 00:53.04 |
| 100th | Julia Hartman-Teske | St. Mary's Academy (Englewood, CO) | Final | | 00:53.54 |
| 101st | Deisy Escalante | Denver West (Denver, CO) | Final | | 00:53.74 |
| 102nd | Marissa Tolmich | Denver South (Denver, CO) | Final | | 00:53.95 |
| 103rd | Erica Clark | Thomas Jefferson (Denver, CO) | Final | | 00:55.25 |
| 104th | Victoria De | Denver South (Denver, CO) | Final | | 00:55.64 |
| 105th | Dejaune Ellerbee | Denver South (Denver, CO) | Final | | 00:55.96 |
| 106th | Haley Rosenberg | Thomas Jefferson (Denver, CO) | Final | | 00:56.73 |
| 107th | Samantha Mejia | Denver North (Denver, CO) | Final | | 00:57.47 |
| 108th | Emily Carillo | Far Northeast Warriors (Denver, CO) | Final | | 00:58.24 |
| 109th | Itzel Morales | Denver South (Denver, CO) | Final | | 00:59.73 |
| 110th | Aileen Alvardo | Lincoln (Denver, CO) | Final | | 01:00.07 |
| 111th | LaRae Ross | Denver West (Denver, CO) | Final | | 01:02.48 |
| 112th | Sylvia Ferrales-Manzanares | Denver North (Denver, CO) | Final | | 01:02.54 |
| 113th | Yathzy Munoz | Denver West (Denver, CO) | Final | | 01:04.81 |
| 114th | Tia Van | Denver West (Denver, CO) | Final | | 01:06.19 |
| 115th | Shukri Hassan | Denver North (Denver, CO) | Final | | 01:08.30 |
| 116th | Liliana Mejia | Lincoln (Denver, CO) | Final | | 01:09.08 |
| 117th | Carolina Perdomo | Far Northeast Warriors (Denver, CO) | Final | | 01:15.49 |
| 118th | Gabby Schmitz | Lincoln (Denver, CO) | Final | | 01:23.29 |
| 119th | Caroline Pena | Far Northeast Warriors (Denver, CO) | Final | | 01:37.52 |
