| 1st | Phoebe Briney | Thomas Jefferson (Denver, CO) | Final | | 00:26.72 |
| 2nd | Annika Abramson | George Washington (Denver, CO) | Final | | 00:27.29 |
| 3rd | Meredith Neid | George Washington (Denver, CO) | Final | | 00:27.32 |
| 4th | Naomi Fry | Denver East (Denver, CO) | Final | | 00:27.49 |
| 5th | Megan George | Thomas Jefferson (Denver, CO) | Final | | 00:27.78 |
| 6th | Payton Zelkin | Denver East (Denver, CO) | Final | | 00:27.89 |
| 7th | Emma Gentry | Denver North (Denver, CO) | Final | | 00:28.14 |
| 8th | Beatrix Kelly | George Washington (Denver, CO) | Final | | 00:28.46 |
| 9th | Sophia Graham | George Washington (Denver, CO) | Final | | 00:28.58 |
| 10th | Charlotte McHughs | George Washington (Denver, CO) | Final | | 00:29.02 |
| 11th | Jenna Bryant | George Washington (Denver, CO) | Final | | 00:29.08 |
| 12th | Erin Harwood | George Washington (Denver, CO) | Final | | 00:29.27 |
| 13th | Isabella Pitt | Thomas Jefferson (Denver, CO) | Final | | 00:29.71 |
| 14th | Kathryn Seaman | George Washington (Denver, CO) | Final | | 00:30.52 |
| 15th | Kaia Tryggestad | Northfield (Denver, CO) | Final | | 00:30.52 |
| 16th | Bella Wentland | Denver North (Denver, CO) | Final | | 00:30.75 |
| 17th | Tillie Pinkowitz | Denver South (Denver, CO) | Final | | 00:30.80 |
| 18th | Amanda Liedtke | George Washington (Denver, CO) | Final | | 00:31.04 |
| 19th | Emilia Moody | Northfield (Denver, CO) | Final | | 00:31.13 |
| 20th | Anna Neznamova | George Washington (Denver, CO) | Final | | 00:31.40 |
| 21st | Mina Combs | George Washington (Denver, CO) | Final | | 00:31.76 |
| 22nd | Anna Winnecke | Denver East (Denver, CO) | Final | | 00:31.82 |
| 23rd | Peren Yilmazturk | Denver East (Denver, CO) | Final | | 00:31.83 |
| 24th | Zia Meyers | George Washington (Denver, CO) | Final | | 00:31.85 |
| 25th | Jane Parney | Denver East (Denver, CO) | Final | | 00:31.92 |
| 26th | Macy Schram | Northfield (Denver, CO) | Final | | 00:31.94 |
| 27th | Madeline Rodgers | Denver East (Denver, CO) | Final | | 00:32.26 |
| 28th | Ursula Larson | George Washington (Denver, CO) | Final | | 00:32.28 |
| 29th | Elizabeth Melton | George Washington (Denver, CO) | Final | | 00:32.41 |
| 30th | Caitlin Gallik | Denver East (Denver, CO) | Final | | 00:32.57 |
| 31st | Grace McClung | Denver South (Denver, CO) | Final | | 00:32.89 |
| 32nd | Addie McLain | Thomas Jefferson (Denver, CO) | Final | | 00:32.92 |
| 33rd | Jimena Cristerna | George Washington (Denver, CO) | Final | | 00:32.96 |
| 34th | Zoe Pettersen | George Washington (Denver, CO) | Final | | 00:33.11 |
| 35th | Amelie Gardner | Northfield (Denver, CO) | Final | | 00:33.13 |
| 36th | Campbell Moore | Denver South (Denver, CO) | Final | | 00:33.40 |
| 37th | Izzy Ellertson | Denver North (Denver, CO) | Final | | 00:33.43 |
| 38th | Piper Ahern | Denver North (Denver, CO) | Final | | 00:33.43 |
| 39th | Faith Toner | Thomas Jefferson (Denver, CO) | Final | | 00:33.48 |
| 40th | Katia Marusich | George Washington (Denver, CO) | Final | | 00:33.56 |
| 41st | Sophia DiMaria | Denver North (Denver, CO) | Final | | 00:33.69 |
| 42nd | Sonja Feierabend | Lincoln (Denver, CO) | Final | | 00:33.73 |
| 43rd | Ashley Mackenzie | Denver North (Denver, CO) | Final | | 00:33.83 |
| 44th | Brady Allison | Denver South (Denver, CO) | Final | | 00:33.88 |
| 45th | Emerson Whitford | George Washington (Denver, CO) | Final | | 00:33.96 |
| 46th | Sawyer Fenton | Denver South (Denver, CO) | Final | | 00:34.01 |
| 47th | Lucy White | Northfield (Denver, CO) | Final | | 00:34.20 |
| 48th | Sadie Johnson | Northfield (Denver, CO) | Final | | 00:34.21 |
| 49th | Elsa Manly | Denver East (Denver, CO) | Final | | 00:34.53 |
| 50th | Mackenzie Kilk | Northfield (Denver, CO) | Final | | 00:34.60 |
| 51st | Priscilla Sanchez | Northfield (Denver, CO) | Final | | 00:34.62 |
| 52nd | Ella Hanisch | George Washington (Denver, CO) | Final | | 00:34.73 |
| 53rd | Allison Yager | Denver East (Denver, CO) | Final | | 00:35.02 |
| 54th | Eris Sanders | George Washington (Denver, CO) | Final | | 00:35.22 |
| 55th | Lucy Caldwell | Denver North (Denver, CO) | Final | | 00:35.28 |
| 56th | Alexandria Emerson | George Washington (Denver, CO) | Final | | 00:35.30 |
| 57th | CeCe Prime-Morales | Thomas Jefferson (Denver, CO) | Final | | 00:35.36 |
| 58th | Veronica James | Denver South (Denver, CO) | Final | | 00:35.39 |
| 59th | Emma Merrer | Northfield (Denver, CO) | Final | | 00:35.51 |
| 60th | Ellie Goldstein | Denver East (Denver, CO) | Final | | 00:35.53 |
| 61st | Aftyn Dewhurst | Denver North (Denver, CO) | Final | | 00:35.53 |
| 62nd | Siveli Santos | Denver East (Denver, CO) | Final | | 00:35.68 |
| 63rd | Evelyn Higgins | Denver East (Denver, CO) | Final | | 00:35.69 |
| 64th | Hallie Lowe | George Washington (Denver, CO) | Final | | 00:35.80 |
| 65th | Brooklin Stromberg | Northfield (Denver, CO) | Final | | 00:36.05 |
| 66th | Nina Dorighi | Denver South (Denver, CO) | Final | | 00:36.22 |
| 67th | Vivian Conway | George Washington (Denver, CO) | Final | | 00:36.30 |
| 68th | Abigail Cunningham | Denver South (Denver, CO) | Final | | 00:36.57 |
| 69th | Maya Kitei | Denver South (Denver, CO) | Final | | 00:36.70 |
| 70th | Sofia Meltzer | Denver South (Denver, CO) | Final | | 00:37.13 |
| 71st | Phemie Lucey | Denver South (Denver, CO) | Final | | 00:37.20 |
| 72nd | Aspen Coates | Denver North (Denver, CO) | Final | | 00:37.38 |
| 73rd | Isabella King | Denver East (Denver, CO) | Final | | 00:37.57 |
| 74th | Sophia Fabian | George Washington (Denver, CO) | Final | | 00:38.12 |
| 75th | Alexandra Maxon | Denver South (Denver, CO) | Final | | 00:38.24 |
| 76th | Lillian McGlothlen | Denver East (Denver, CO) | Final | | 00:38.29 |
| 77th | Alex Stone | Denver North (Denver, CO) | Final | | 00:38.35 |
| 78th | Kaleigh Johnston | Denver South (Denver, CO) | Final | | 00:38.64 |
| 79th | Marlo Pearson | Northfield (Denver, CO) | Final | | 00:38.80 |
| 80th | Hannah Rossow | Denver South (Denver, CO) | Final | | 00:38.91 |
| 81st | Amelia Carson | Northfield (Denver, CO) | Final | | 00:39.01 |
| 82nd | Claire Miller | Northfield (Denver, CO) | Final | | 00:39.01 |
| 83rd | Amy Harling | George Washington (Denver, CO) | Final | | 00:39.03 |
| 84th | Kayla Kilk | Northfield (Denver, CO) | Final | | 00:39.58 |
| 85th | Samantha Baker | Thomas Jefferson (Denver, CO) | Final | | 00:40.75 |
| 86th | Sofia Davidson | George Washington (Denver, CO) | Final | | 00:40.79 |
| 87th | Marissa Mohabir | Denver South (Denver, CO) | Final | | 00:41.42 |
| 88th | Margo Ladis | George Washington (Denver, CO) | Final | | 00:41.48 |
| 89th | Lana Combs | George Washington (Denver, CO) | Final | | 00:41.56 |
| 90th | Sarah Asres | Denver East (Denver, CO) | Final | | 00:41.78 |
| 91st | Aalyiah Quintana | Denver South (Denver, CO) | Final | | 00:41.81 |
| 92nd | Maya Williams | Denver East (Denver, CO) | Final | | 00:41.98 |
| 93rd | Mia Ferrero-Lampron | George Washington (Denver, CO) | Final | | 00:42.18 |
| 94th | Kristal Phelps | Lincoln (Denver, CO) | Final | | 00:42.24 |
| 95th | Eloise McGoldrick | Denver East (Denver, CO) | Final | | 00:42.25 |
| 96th | Katie Murrin | Northfield (Denver, CO) | Final | | 00:42.62 |
| 97th | Katharine Rigsby | George Washington (Denver, CO) | Final | | 00:42.69 |
| 98th | Savannah Pitt | Thomas Jefferson (Denver, CO) | Final | | 00:43.40 |
| 99th | Genessi Hernandez | Thomas Jefferson (Denver, CO) | Final | | 00:43.51 |
| 100th | Willa Conway | George Washington (Denver, CO) | Final | | 00:43.63 |
| 101st | Mary Jarecke | Thomas Jefferson (Denver, CO) | Final | | 00:43.68 |
| 102nd | Natalie Aragon | Lincoln (Denver, CO) | Final | | 00:43.78 |
| 103rd | Sarah Jackson | Northfield (Denver, CO) | Final | | 00:43.81 |
| 104th | Starr Bianchi | Denver North (Denver, CO) | Final | | 00:44.13 |
| 105th | Natalie Dominguez | Lincoln (Denver, CO) | Final | | 00:44.21 |
| 106th | Sabdi Pulido | Denver South (Denver, CO) | Final | | 00:44.38 |
| 107th | Grace Davis | George Washington (Denver, CO) | Final | | 00:45.11 |
| 108th | Kendal Gomez | Denver East (Denver, CO) | Final | | 00:45.37 |
| 109th | Lauren Sudmeier | Denver East (Denver, CO) | Final | | 00:45.38 |
| 110th | Peggy Ho | Thomas Jefferson (Denver, CO) | Final | | 00:45.60 |
| 111th | Tess White | Northfield (Denver, CO) | Final | | 00:45.71 |
| 112th | Zulema Herdandez-Cruz | Lincoln (Denver, CO) | Final | | 00:45.79 |
| 113th | Delani Arteaga | Denver South (Denver, CO) | Final | | 00:46.38 |
| 114th | Jackseny Roldan | Lincoln (Denver, CO) | Final | | 00:47.47 |
| 115th | Madeleine Taylor | Denver South (Denver, CO) | Final | | 00:47.65 |
| 116th | Amelia Sun | George Washington (Denver, CO) | Final | | 00:47.78 |
| 117th | Caroline Brown | Denver South (Denver, CO) | Final | | 00:48.92 |
| 118th | Arion Gray | Northfield (Denver, CO) | Final | | 00:48.93 |
| 119th | Gracie Nunez | Denver East (Denver, CO) | Final | | 00:50.47 |
| 120th | Samantha Castro | Thomas Jefferson (Denver, CO) | Final | | 00:51.95 |
| 121st | Jazim Espinoza | Lincoln (Denver, CO) | Final | | 01:00.61 |
| 122nd | Savannah Whatley | Lincoln (Denver, CO) | Final | | 01:01.42 |
