| 1st | Abigail Gardner | Rock Canyon (Highlands Ranch, CO) | Final | | 00:25.97 |
| 2nd | Lenja Osborne | Rock Canyon (Highlands Ranch, CO) | Final | | 00:26.19 |
| 3rd | Morgan Edstrom | Rock Canyon (Highlands Ranch, CO) | Final | | 00:26.21 |
| 4th | Rebecca Hildebrand | Grandview (Aurora, CO) | Final | | 00:26.29 |
| 5th | Megan Doubrava | Grandview (Aurora, CO) | Final | | 00:26.52 |
| 6th | Ella McGonigle | Chaparral (Parker, CO) | Final | | 00:26.70 |
| 7th | Ava Zadigian | Grandview (Aurora, CO) | Final | | 00:26.73 |
| 8th | Jaime Toomer | Chaparral (Parker, CO) | Final | | 00:26.74 |
| 9th | Peyton Belcher | Grandview (Aurora, CO) | Final | | 00:26.77 |
| 10th | Ava McGonigle | Chaparral (Parker, CO) | Final | | 00:26.80 |
| 11th | Chayse Schneider | Chaparral (Parker, CO) | Final | | 00:27.13 |
| 12th | Madeline Alley | Rock Canyon (Highlands Ranch, CO) | Final | | 00:27.40 |
| 13th | Brooke Michael | Chaparral (Parker, CO) | Final | | 00:27.40 |
| 14th | Madelyn Harris | Rock Canyon (Highlands Ranch, CO) | Final | | 00:27.43 |
| 15th | Liv Higgins | Grandview (Aurora, CO) | Final | | 00:27.84 |
| 16th | Avery Tomlinson | Rock Canyon (Highlands Ranch, CO) | Final | | 00:27.91 |
| 17th | Serena Thomas | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.01 |
| 18th | Kaitlyn Hafer | Grandview (Aurora, CO) | Final | | 00:28.04 |
| 19th | Rylee Kerekes | Chaparral (Parker, CO) | Final | | 00:28.23 |
| 20th | Maya Amerlan | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.32 |
| 21st | Paige Dailey | Grandview (Aurora, CO) | Final | | 00:28.33 |
| 22nd | Ella May | Chaparral (Parker, CO) | Final | | 00:28.36 |
| 23rd | Anna Carpenter | Grandview (Aurora, CO) | Final | | 00:28.43 |
| 24th | Katie Fleming | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.64 |
| 25th | AC Hart | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.64 |
| 26th | Elise Barton | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.76 |
| 27th | Abigail McDaniel | Grandview (Aurora, CO) | Final | | 00:28.95 |
| 28th | Meredith Mounier | Grandview (Aurora, CO) | Final | | 00:29.28 |
| 29th | Anastasiya Fajda | Grandview (Aurora, CO) | Final | | 00:29.32 |
| 30th | Sophia Lush | Rock Canyon (Highlands Ranch, CO) | Final | | 00:29.38 |
| 31st | Nicole Damian-Campbell | Rock Canyon (Highlands Ranch, CO) | Final | | 00:29.43 |
| 32nd | Adrienne Lam | Grandview (Aurora, CO) | Final | | 00:29.51 |
| 33rd | Ella Deitz | Rock Canyon (Highlands Ranch, CO) | Final | | 00:29.53 |
| 34th | Kateryna Fajda | Grandview (Aurora, CO) | Final | | 00:29.58 |
| 35th | Maya Vora | Rock Canyon (Highlands Ranch, CO) | Final | | 00:29.75 |
| 36th | Brooklyn Stevens | Grandview (Aurora, CO) | Final | | 00:29.79 |
| 37th | Taylor Schmahl | Rock Canyon (Highlands Ranch, CO) | Final | | 00:29.80 |
| 38th | Noella Lopez | Chaparral (Parker, CO) | Final | | 00:29.87 |
| 39th | Camden Krumholz | Arapahoe (Centennial, CO) | Final | | 00:29.93 |
| 40th | Kaeley Elevich | Rock Canyon (Highlands Ranch, CO) | Final | | 00:30.28 |
| 41st | Frances Wyman | Chaparral (Parker, CO) | Final | | 00:30.31 |
| 42nd | Kennedy Jeppson | Rock Canyon (Highlands Ranch, CO) | Final | | 00:30.50 |
| 43rd | Lindsay Scott | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.03 |
| 44th | Emily Brundage | Chaparral (Parker, CO) | Final | | 00:31.11 |
| 45th | Elena Gammel-Perera | Chaparral (Parker, CO) | Final | | 00:31.40 |
| 46th | Marley Johnston | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.49 |
| 47th | Elizabeth Lemeshewsky | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.51 |
| 48th | Ainsley Murchison | Grandview (Aurora, CO) | Final | | 00:31.53 |
| 49th | Arley Stark | Arapahoe (Centennial, CO) | Final | | 00:31.74 |
| 50th | Nora Hopfer | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.92 |
| 51st | Alyssa Cook | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.98 |
| 52nd | Katherine Lindig | Arapahoe (Centennial, CO) | Final | | 00:32.01 |
| 53rd | Kaylee Ellsworth | Chaparral (Parker, CO) | Final | | 00:32.04 |
| 54th | Lydia Nichols | Arapahoe (Centennial, CO) | Final | | 00:32.06 |
| 55th | Samantha Sykes | Grandview (Aurora, CO) | Final | | 00:32.07 |
| 56th | Kira McCulloch | Grandview (Aurora, CO) | Final | | 00:32.13 |
| 57th | Sydney Lockett | Chaparral (Parker, CO) | Final | | 00:32.86 |
| 58th | Gianna Wolter-Conti | Chaparral (Parker, CO) | Final | | 00:33.10 |
| 59th | Karli Holmes | Arapahoe (Centennial, CO) | Final | | 00:33.13 |
| 60th | Claire Price | Rock Canyon (Highlands Ranch, CO) | Final | | 00:33.27 |
| 61st | Adeline Goujon | Rock Canyon (Highlands Ranch, CO) | Final | | 00:33.70 |
| 62nd | Kayla Romero | Arapahoe (Centennial, CO) | Final | | 00:34.07 |
| 63rd | Anna Peecher | Arapahoe (Centennial, CO) | Final | | 00:34.28 |
| 64th | Lily Kroger | Grandview (Aurora, CO) | Final | | 00:34.65 |
| 65th | Paige Rogers | Rock Canyon (Highlands Ranch, CO) | Final | | 00:34.80 |
| 66th | Sofia Chakounis | Arapahoe (Centennial, CO) | Final | | 00:34.88 |
| 67th | Neha Barani | Rock Canyon (Highlands Ranch, CO) | Final | | 00:35.08 |
| 68th | Allison Billings | Chaparral (Parker, CO) | Final | | 00:35.10 |
| 69th | Navika Thomas | Rock Canyon (Highlands Ranch, CO) | Final | | 00:36.38 |
| 70th | Hannah Redfearn | Chaparral (Parker, CO) | Final | | 00:36.40 |
| 71st | Phoebe Seager | Rock Canyon (Highlands Ranch, CO) | Final | | 00:36.88 |
| 72nd | Sienna Collins | Rock Canyon (Highlands Ranch, CO) | Final | | 00:37.09 |
| 73rd | Dhanishta Mattoo | Rock Canyon (Highlands Ranch, CO) | Final | | 00:41.83 |
| 74th | Brecken Cartwright | Arapahoe (Centennial, CO) | Final | | 00:42.37 |
| 75th | Annoushka Niunge | Rock Canyon (Highlands Ranch, CO) | Final | | 00:44.87 |
| 76th | Mahi Chalicham | Rock Canyon (Highlands Ranch, CO) | Final | | 00:48.90 |
| 77th | Ava Frederick | Rock Canyon (Highlands Ranch, CO) | Final | | 01:02.54 |
