| 1st | Claire Neid | George Washington (Denver, CO) | Final | | 00:26.35 |
| 2nd | Kendra Clary | George Washington (Denver, CO) | Final | | 00:26.60 |
| 3rd | Ava Baumann | Northfield (Denver, CO) | Final | | 00:27.74 |
| 4th | Hannah O'Neil | Denver East (Denver, CO) | Final | | 00:28.09 |
| 5th | Abigail Seman | Denver East (Denver, CO) | Final | | 00:28.36 |
| 6th | Christina Rosales | Lincoln (Denver, CO) | Final | | 00:29.25 |
| 7th | Emma Obrien | Denver South (Denver, CO) | Final | | 00:29.26 |
| 8th | Mia Nelson | Denver East (Denver, CO) | Final | | 00:29.38 |
| 9th | Laine Draper | George Washington (Denver, CO) | Final | | 00:29.41 |
| 10th | Halie Hall | Thomas Jefferson (Denver, CO) | Final | | 00:29.43 |
| 11th | J'Nai Lawrence | Denver East (Denver, CO) | Final | | 00:29.64 |
| 12th | Meredith Neid | George Washington (Denver, CO) | Final | | 00:29.87 |
| 13th | Schuyler Nicholson | Denver East (Denver, CO) | Final | | 00:30.24 |
| 14th | Kathryn Seaman | George Washington (Denver, CO) | Final | | 00:30.66 |
| 15th | Cadence Litt | George Washington (Denver, CO) | Final | | 00:30.66 |
| 16th | Abigail Bosworth | Denver West (Denver, CO) | Final | | 00:30.81 |
| 17th | Emily Harrison | Denver East (Denver, CO) | Final | | 00:31.05 |
| 18th | Sophia Ward | Thomas Jefferson (Denver, CO) | Final | | 00:31.05 |
| 19th | Elise Ackley | Denver East (Denver, CO) | Final | | 00:31.75 |
| 20th | Clara Johnston | Denver East (Denver, CO) | Final | | 00:31.91 |
| 21st | Hannah Hall | Denver South (Denver, CO) | Final | | 00:32.06 |
| 22nd | Berkley Larson | George Washington (Denver, CO) | Final | | 00:32.20 |
| 23rd | Pippi Thompson | Denver South (Denver, CO) | Final | | 00:32.21 |
| 24th | Kennedy Austin | Denver East (Denver, CO) | Final | | 00:32.67 |
| 25th | Chloe Applegate | Denver South (Denver, CO) | Final | | 00:33.00 |
| 26th | Lindsey Kubo | Denver East (Denver, CO) | Final | | 00:33.01 |
| 27th | Judith Dietz | Lincoln (Denver, CO) | Final | | 00:33.15 |
| 28th | Gabriella Rodriguez | Denver East (Denver, CO) | Final | | 00:33.20 |
| 29th | Payton Brown | Denver East (Denver, CO) | Final | | 00:33.38 |
| 30th | Emily Lear | Denver South (Denver, CO) | Final | | 00:33.65 |
| 31st | Melina Kilen | Denver North (Denver, CO) | Final | | 00:33.77 |
| 32nd | Nancy Bowdouris | Denver East (Denver, CO) | Final | | 00:33.89 |
| 33rd | Lindsey Neukirch | Denver East (Denver, CO) | Final | | 00:34.01 |
| 34th | Chava Baldiva | Denver South (Denver, CO) | Final | | 00:34.17 |
| 35th | Ashley McCord | Lincoln (Denver, CO) | Final | | 00:34.23 |
| 36th | Katherine Kendall | Denver East (Denver, CO) | Final | | 00:34.37 |
| 37th | Hillary Holcomb | Thomas Jefferson (Denver, CO) | Final | | 00:34.43 |
| 38th | Lucy LoJacono | Thomas Jefferson (Denver, CO) | Final | | 00:34.55 |
| 39th | Lily Jorgensen | Denver East (Denver, CO) | Final | | 00:35.07 |
| 40th | Lauren Pulver | Thomas Jefferson (Denver, CO) | Final | | 00:35.15 |
| 41st | Kailey Silverberg | Denver South (Denver, CO) | Final | | 00:35.33 |
| 42nd | Reece Dines | Thomas Jefferson (Denver, CO) | Final | | 00:35.66 |
| 43rd | Jennifer Rutledge | Denver East (Denver, CO) | Final | | 00:35.82 |
| 44th | Anne Welch | Denver South (Denver, CO) | Final | | 00:36.14 |
| 45th | Riley Mower | Denver South (Denver, CO) | Final | | 00:36.25 |
| 46th | Piper Molins | Denver East (Denver, CO) | Final | | 00:36.49 |
| 47th | Sydni Garica | Lincoln (Denver, CO) | Final | | 00:36.55 |
| 48th | Alina Gallego | George Washington (Denver, CO) | Final | | 00:36.58 |
| 49th | Haley Paterson | Denver South (Denver, CO) | Final | | 00:36.83 |
| 50th | Phoebe Fanganello | Denver East (Denver, CO) | Final | | 00:36.83 |
| 51st | Annika Pavlik | Denver East (Denver, CO) | Final | | 00:36.87 |
| 52nd | Faith Toner | Thomas Jefferson (Denver, CO) | Final | | 00:37.02 |
| 53rd | Julia Gonzales | George Washington (Denver, CO) | Final | | 00:37.14 |
| 54th | Mariana Ledezma | Lincoln (Denver, CO) | Final | | 00:37.22 |
| 55th | Ruby Maldanado | George Washington (Denver, CO) | Final | | 00:37.49 |
| 56th | Gabriela Lopez | Lincoln (Denver, CO) | Final | | 00:37.59 |
| 57th | Isabella Wentland | Denver North (Denver, CO) | Final | | 00:37.77 |
| 58th | Chloe Robinson | Denver South (Denver, CO) | Final | | 00:38.18 |
| 59th | Zoe Pettersen | George Washington (Denver, CO) | Final | | 00:38.26 |
| 60th | Elena Miranda-Hernandez | Northfield (Denver, CO) | Final | | 00:38.53 |
| 61st | Mattie Randall | Thomas Jefferson (Denver, CO) | Final | | 00:38.56 |
| 62nd | Mackenzie Kilk | Northfield (Denver, CO) | Final | | 00:38.58 |
| 63rd | Cassandra Calhoun | Denver South (Denver, CO) | Final | | 00:38.82 |
| 64th | Christy Vo | Denver South (Denver, CO) | Final | | 00:39.00 |
| 65th | Scout Hope | Denver West (Denver, CO) | Final | | 00:39.10 |
| 66th | Emma Fiedler | Denver East (Denver, CO) | Final | | 00:39.55 |
| 67th | Gabrielle Day | Denver East (Denver, CO) | Final | | 00:39.67 |
| 68th | Serena Buscarello | Denver South (Denver, CO) | Final | | 00:39.76 |
| 69th | Jessica Plummer | Denver South (Denver, CO) | Final | | 00:39.88 |
| 70th | Lily Dailey | Northfield (Denver, CO) | Final | | 00:40.12 |
| 71st | Margaret Goff | Denver South (Denver, CO) | Final | | 00:40.17 |
| 72nd | Kendall Reed | George Washington (Denver, CO) | Final | | 00:40.56 |
| 73rd | Addi Schwieterman | George Washington (Denver, CO) | Final | | 00:41.49 |
| 74th | Emmylou Williams | Denver South (Denver, CO) | Final | | 00:41.55 |
| 75th | Lucia Garramone | Denver South (Denver, CO) | Final | | 00:41.92 |
| 76th | Ella Kudzma | Denver South (Denver, CO) | Final | | 00:42.91 |
| 77th | Emma Rasmussen | George Washington (Denver, CO) | Final | | 00:44.37 |
| 78th | Ashley Vargas | Lincoln (Denver, CO) | Final | | 00:44.40 |
| 79th | Miriam Primus | Thomas Jefferson (Denver, CO) | Final | | 00:44.68 |
| 80th | Abigail Garcia | Denver North (Denver, CO) | Final | | 00:45.56 |
| 81st | Frances Vega | Denver East (Denver, CO) | Final | | 00:45.70 |
| 82nd | Breinn Gray | Northfield (Denver, CO) | Final | | 00:47.27 |
| 83rd | Brittany Davila | George Washington (Denver, CO) | Final | | 00:47.60 |
| 84th | Kate Dominguez | Northfield (Denver, CO) | Final | | 00:50.98 |
| 85th | Alina Lopez | Denver West (Denver, CO) | Final | | 00:51.24 |
| 86th | Farinaz Molaei | George Washington (Denver, CO) | Final | | 00:56.13 |
| 87th | Lidia Perez | Denver West (Denver, CO) | Final | | 00:56.32 |
| 88th | Shania Quintana | Denver West (Denver, CO) | Final | | 00:58.10 |
| 89th | Krist Tran | Lincoln (Denver, CO) | Final | | 00:59.56 |
| 90th | Courtney Sabal | Northfield (Denver, CO) | Final | | 01:26.25 |
