| 1st | Halie Hall | Thomas Jefferson (Denver, CO) | Final | | 00:29.39 |
| 2nd | Lucca Raabe | George Washington (Denver, CO) | Final | | 00:29.47 |
| 3rd | Rachell Brandorff | Thomas Jefferson (Denver, CO) | Final | | 00:29.57 |
| 4th | Gabby Camp-Lagueux | George Washington (Denver, CO) | Final | | 00:29.84 |
| 5th | Camy Guenther | Thomas Jefferson (Denver, CO) | Final | | 00:30.50 |
| 6th | Kathryn Beegle | Denver South (Denver, CO) | Final | | 00:30.56 |
| 7th | Annie Welch | George Washington (Denver, CO) | Final | | 00:31.03 |
| 8th | Sophia Ward | Thomas Jefferson (Denver, CO) | Final | | 00:31.15 |
| 9th | Margaret Torgerson | Denver East (Denver, CO) | Final | | 00:31.75 |
| 10th | Olivia Fullerton | Thomas Jefferson (Denver, CO) | Final | | 00:32.21 |
| 11th | Lexanne Stegeman | Denver South (Denver, CO) | Final | | 00:32.56 |
| 12th | Victoria Valladares | Denver West (Denver, CO) | Final | | 00:32.75 |
| 13th | Kaelyne Gomez | Denver East (Denver, CO) | Final | | 00:33.05 |
| 14th | Emily Main | Lincoln (Denver, CO) | Final | | 00:33.23 |
| 15th | Lauren Pulver | Thomas Jefferson (Denver, CO) | Final | | 00:33.88 |
| 16th | Avery Williams | George Washington (Denver, CO) | Final | | 00:34.40 |
| 17th | Lameine VanDerMeij | Denver East (Denver, CO) | Final | | 00:34.87 |
| 18th | Deanna Main | Lincoln (Denver, CO) | Final | | 00:34.91 |
| 19th | Ashley McCord | Lincoln (Denver, CO) | Final | | 00:35.52 |
| 20th | Sydni Garica | Lincoln (Denver, CO) | Final | | 00:35.69 |
| 21st | Gabriela Lopez | Lincoln (Denver, CO) | Final | | 00:35.73 |
| 22nd | Mariana Ledezma | Lincoln (Denver, CO) | Final | | 00:36.14 |
| 23rd | Marie Howard | George Washington (Denver, CO) | Final | | 00:36.69 |
| 24th | Alina Gallegos | George Washington (Denver, CO) | Final | | 00:36.81 |
| 25th | Sarah Davies-Schley | Denver North (Denver, CO) | Final | | 00:37.24 |
| 26th | Audrey Wilhite | Lincoln (Denver, CO) | Final | | 00:37.75 |
| 27th | Anna Heimel | George Washington (Denver, CO) | Final | | 00:37.90 |
| 28th | Camille Moore | Thomas Jefferson (Denver, CO) | Final | | 00:37.90 |
| 29th | Sarah Maher | Denver North (Denver, CO) | Final | | 00:38.42 |
| 30th | Meryn Johnson | Denver West (Denver, CO) | Final | | 00:38.61 |
| 31st | Unique Trujillo | Denver West (Denver, CO) | Final | | 00:39.08 |
| 32nd | Annika Pavlik | Denver East (Denver, CO) | Final | | 00:39.19 |
| 33rd | Isabelle Ryan | Thomas Jefferson (Denver, CO) | Final | | 00:39.25 |
| 34th | Asiane Jackson | Thomas Jefferson (Denver, CO) | Final | | 00:40.00 |
| 35th | Courtney Davis | Denver South (Denver, CO) | Final | | 00:41.82 |
| 36th | Gnaleh Kande | Far Northeast Warriors (Denver, CO) | Final | | 00:42.26 |
| 37th | Nancy Rodriguez | Thomas Jefferson (Denver, CO) | Final | | 00:42.39 |
| 38th | Aileen Alvardo | Lincoln (Denver, CO) | Final | | 00:43.04 |
| 39th | Elizabeth Banuelos | Lincoln (Denver, CO) | Final | | 00:43.14 |
| 40th | Alondra Arreola | Far Northeast Warriors (Denver, CO) | Final | | 00:43.32 |
| 41st | Victoria De | Denver South (Denver, CO) | Final | | 00:44.32 |
| 42nd | Renee Aburto | Thomas Jefferson (Denver, CO) | Final | | 00:44.42 |
| 43rd | Emily Carillo | Far Northeast Warriors (Denver, CO) | Final | | 00:44.46 |
| 44th | Lindsey Connolly | Denver West (Denver, CO) | Final | | 00:45.72 |
| 45th | Jade Jiron | Denver North (Denver, CO) | Final | | 00:45.75 |
| 46th | Cristal Lobo | Denver West (Denver, CO) | Final | | 00:45.92 |
| 47th | Jammy Johnson | Denver West (Denver, CO) | Final | | 00:46.02 |
| 48th | Getssemany Rivera | Lincoln (Denver, CO) | Final | | 00:46.31 |
| 49th | Gabriela Mejia | Denver North (Denver, CO) | Final | | 00:46.79 |
| 50th | Abigail Garcia | Denver North (Denver, CO) | Final | | 00:46.83 |
| 51st | Julissa Leal | Denver West (Denver, CO) | Final | | 00:47.34 |
| 52nd | Binh Nguyen | Denver North (Denver, CO) | Final | | 00:47.81 |
| 53rd | Alexis Hogan | Denver West (Denver, CO) | Final | | 00:48.15 |
| 54th | Deisy Escalante | Denver West (Denver, CO) | Final | | 00:49.25 |
| 55th | Haley Rosenberg | Thomas Jefferson (Denver, CO) | Final | | 00:49.52 |
| 56th | Alina Lopez | Denver West (Denver, CO) | Final | | 00:50.84 |
| 57th | Yathzy Munoz | Denver West (Denver, CO) | Final | | 00:51.52 |
| 58th | Liliana Mejia | Lincoln (Denver, CO) | Final | | 00:52.77 |
| 59th | Tia Van | Denver West (Denver, CO) | Final | | 00:54.27 |
| 60th | Shukri Hassan | Denver North (Denver, CO) | Final | | 00:55.22 |
| 61st | Gabby Schmitz | Lincoln (Denver, CO) | Final | | 01:08.20 |
