| 1st | Emily Kahn | George Washington (Denver, CO) | Final | | 00:25.99 |
| 2nd | Phoebe Briney | Thomas Jefferson (Denver, CO) | Final | | 00:26.60 |
| 3rd | Annika Abramson | George Washington (Denver, CO) | Final | | 00:27.10 |
| 4th | Morgan George | Thomas Jefferson (Denver, CO) | Final | | 00:27.34 |
| 5th | Meredith Neid | George Washington (Denver, CO) | Final | | 00:27.51 |
| 6th | Caroline Rogers | Denver East (Denver, CO) | Final | | 00:28.24 |
| 7th | Abigail Seman | Denver East (Denver, CO) | Final | | 00:28.53 |
| 8th | Sonya Hay | Denver East (Denver, CO) | Final | | 00:28.55 |
| 9th | Hanna Channell | Thomas Jefferson (Denver, CO) | Final | | 00:28.81 |
| 10th | Caroline Kundahl | Denver East (Denver, CO) | Final | | 00:29.09 |
| 11th | Natalie Falta | Denver East (Denver, CO) | Final | | 00:29.42 |
| 12th | Lily O'Brien | Denver East (Denver, CO) | Final | | 00:29.88 |
| 13th | Elise Ackley | Denver East (Denver, CO) | Final | | 00:29.99 |
| 14th | Ella Eichberg | Denver East (Denver, CO) | Final | | 00:29.99 |
| 15th | Lydia Loof | George Washington (Denver, CO) | Final | | 00:30.02 |
| 16th | Beatrix Kelly | George Washington (Denver, CO) | Final | | 00:30.45 |
| 17th | Katharine Kundahl | Denver East (Denver, CO) | Final | | 00:30.58 |
| 18th | Erin Harwood | George Washington (Denver, CO) | Final | | 00:30.66 |
| 19th | Juju Read | Denver East (Denver, CO) | Final | | 00:30.68 |
| 20th | Kate McVey | Denver East (Denver, CO) | Final | | 00:30.69 |
| 21st | Berkley Larson | George Washington (Denver, CO) | Final | | 00:30.73 |
| 22nd | Georgia Hartley | Thomas Jefferson (Denver, CO) | Final | | 00:30.77 |
| 23rd | Isabella Wentland | Denver North (Denver, CO) | Final | | 00:30.88 |
| 24th | Aislinn Thompson | Denver South (Denver, CO) | Final | | 00:31.06 |
| 25th | EvaMarie Kropp | Denver East (Denver, CO) | Final | | 00:31.24 |
| 26th | Khaki Sawyer | Denver East (Denver, CO) | Final | | 00:31.27 |
| 27th | Margaret Torgerson | Denver East (Denver, CO) | Final | | 00:31.31 |
| 28th | Grace Sadowski | Denver East (Denver, CO) | Final | | 00:31.39 |
| 29th | Maria Davis | Denver East (Denver, CO) | Final | | 00:31.44 |
| 30th | Mina Combs | George Washington (Denver, CO) | Final | | 00:31.92 |
| 31st | Madeline Todd | Denver East (Denver, CO) | Final | | 00:32.00 |
| 32nd | Samantha Bond | George Washington (Denver, CO) | Final | | 00:32.04 |
| 33rd | Lindsey Kubo | Denver East (Denver, CO) | Final | | 00:32.17 |
| 34th | Bailey Nickols | Denver East (Denver, CO) | Final | | 00:32.20 |
| 35th | Kate Adams | Denver North (Denver, CO) | Final | | 00:32.34 |
| 36th | Lucy LoJacono | Thomas Jefferson (Denver, CO) | Final | | 00:32.42 |
| 37th | Sarah Hebbard | Denver East (Denver, CO) | Final | | 00:32.43 |
| 38th | Tillie Pinkowitz | Denver South (Denver, CO) | Final | | 00:32.48 |
| 39th | Lilly Lemme | Denver North (Denver, CO) | Final | | 00:32.50 |
| 40th | Meredith Rigsby | George Washington (Denver, CO) | Final | | 00:32.57 |
| 41st | Chloe Bradford | Denver East (Denver, CO) | Final | | 00:32.67 |
| 42nd | Clara Lorenzo | Denver East (Denver, CO) | Final | | 00:32.68 |
| 43rd | Gabriella Rodriguez | Denver East (Denver, CO) | Final | | 00:32.68 |
| 44th | Kaileigh Parks | Denver East (Denver, CO) | Final | | 00:32.83 |
| 45th | Sonja Feierabend | Lincoln (Denver, CO) | Final | | 00:32.86 |
| 46th | Gabriela Lopez | Lincoln (Denver, CO) | Final | | 00:32.95 |
| 47th | Amelia Wanbo | George Washington (Denver, CO) | Final | | 00:33.04 |
| 48th | Ursula Larson | George Washington (Denver, CO) | Final | | 00:33.05 |
| 49th | Ella Turner | George Washington (Denver, CO) | Final | | 00:33.18 |
| 50th | Payton Goodwin | George Washington (Denver, CO) | Final | | 00:33.25 |
| 51st | Sophie Koenig | Denver East (Denver, CO) | Final | | 00:33.35 |
| 52nd | Amalia Ybarra-Bonner | Thomas Jefferson (Denver, CO) | Final | | 00:33.50 |
| 53rd | Adrienne Schwieterman | George Washington (Denver, CO) | Final | | 00:33.71 |
| 54th | Faith Toner | Thomas Jefferson (Denver, CO) | Final | | 00:33.80 |
| 55th | Amelie Gardner | Northfield (Denver, CO) | Final | | 00:33.92 |
| 56th | Cora Galpern | George Washington (Denver, CO) | Final | | 00:33.96 |
| 57th | Jane Parney | Denver East (Denver, CO) | Final | | 00:33.97 |
| 58th | Sofia Joucovsky | Denver East (Denver, CO) | Final | | 00:34.04 |
| 59th | Sydni Garica | Lincoln (Denver, CO) | Final | | 00:34.16 |
| 60th | Aftyn Dewhurst | Denver North (Denver, CO) | Final | | 00:34.35 |
| 61st | Gina Parker | Denver East (Denver, CO) | Final | | 00:34.43 |
| 62nd | Peren Yilmazturk | Denver East (Denver, CO) | Final | | 00:34.52 |
| 63rd | Anya Overby | Denver East (Denver, CO) | Final | | 00:34.53 |
| 64th | Olivia Buckmelter | Denver East (Denver, CO) | Final | | 00:34.77 |
| 65th | Allison Yager | Denver East (Denver, CO) | Final | | 00:34.80 |
| 66th | Caitlin Gallik | Denver East (Denver, CO) | Final | | 00:34.92 |
| 67th | Brady Allison | Denver South (Denver, CO) | Final | | 00:35.21 |
| 68th | Zia Meyers | George Washington (Denver, CO) | Final | | 00:35.64 |
| 69th | Natalie Ault | George Washington (Denver, CO) | Final | | 00:35.66 |
| 70th | Sara Lusk | Lincoln (Denver, CO) | Final | | 00:35.88 |
| 71st | Isabel Madrid | Denver North (Denver, CO) | Final | | 00:36.26 |
| 72nd | Alexis Ramirez | Denver South (Denver, CO) | Final | | 00:36.43 |
| 73rd | Maya Padilla | Denver North (Denver, CO) | Final | | 00:36.46 |
| 74th | Sawyer Fenton | Denver South (Denver, CO) | Final | | 00:36.86 |
| 75th | Lucy White | Northfield (Denver, CO) | Final | | 00:37.37 |
| 76th | Katie Paul | Northfield (Denver, CO) | Final | | 00:37.45 |
| 77th | Morrhyssey Benz | Thomas Jefferson (Denver, CO) | Final | | 00:37.69 |
| 78th | Vivian Conway | George Washington (Denver, CO) | Final | | 00:37.85 |
| 79th | Emma Hoeschler | Denver South (Denver, CO) | Final | | 00:37.86 |
| 80th | Margaret Goff | Denver South (Denver, CO) | Final | | 00:37.98 |
| 81st | Aspen Coates | Denver North (Denver, CO) | Final | | 00:38.10 |
| 82nd | CeCe Prime-Morales | Thomas Jefferson (Denver, CO) | Final | | 00:38.23 |
| 83rd | Bailey Sheldon | George Washington (Denver, CO) | Final | | 00:38.51 |
| 84th | Camila Cruz Padilla | Thomas Jefferson (Denver, CO) | Final | | 00:38.56 |
| 85th | Ami Pritchett | Lincoln (Denver, CO) | Final | | 00:38.84 |
| 86th | Julieta Vargas | Lincoln (Denver, CO) | Final | | 00:38.87 |
| 87th | Noelani Rael | Denver North (Denver, CO) | Final | | 00:38.89 |
| 88th | Haley Ekstrand | Denver South (Denver, CO) | Final | | 00:38.91 |
| 89th | Siveli Santos | Denver East (Denver, CO) | Final | | 00:39.50 |
| 90th | Mikayla Horton | Northfield (Denver, CO) | Final | | 00:39.65 |
| 91st | Carlene Beaton | Denver North (Denver, CO) | Final | | 00:40.11 |
| 92nd | Zoe Kerr | Denver North (Denver, CO) | Final | | 00:40.24 |
| 93rd | Mylee Martinez | Denver North (Denver, CO) | Final | | 00:41.04 |
| 94th | Margo Ladis | George Washington (Denver, CO) | Final | | 00:41.60 |
| 95th | Ashlyn Kolenc | Denver North (Denver, CO) | Final | | 00:41.65 |
| 96th | Lucy Caldwell | Denver North (Denver, CO) | Final | | 00:41.85 |
| 97th | Veronica James | Denver South (Denver, CO) | Final | | 00:43.37 |
| 98th | Allyson Jones | George Washington (Denver, CO) | Final | | 00:43.55 |
| 99th | Anastasiya Garcia | Denver North (Denver, CO) | Final | | 00:43.66 |
| 100th | Payton Stoll | Northfield (Denver, CO) | Final | | 00:43.83 |
| 101st | Reyna Baldwin | Thomas Jefferson (Denver, CO) | Final | | 00:44.22 |
| 102nd | Isabella Ellertson | Denver North (Denver, CO) | Final | | 00:44.39 |
| 103rd | Madison Ordonez | Denver East (Denver, CO) | Final | | 00:44.47 |
| 104th | Marissa Mohabir | Denver South (Denver, CO) | Final | | 00:44.68 |
| 105th | Ashley Robinson | Denver North (Denver, CO) | Final | | 00:44.88 |
| 106th | Alice Ornelas | Denver North (Denver, CO) | Final | | 00:45.01 |
| 107th | Laney Martinez | Thomas Jefferson (Denver, CO) | Final | | 00:46.13 |
| 108th | Elena Schleuger | Denver East (Denver, CO) | Final | | 00:46.53 |
| 109th | Tyhaunnie Harper | Denver North (Denver, CO) | Final | | 00:46.74 |
| 110th | Farine Azari | Thomas Jefferson (Denver, CO) | Final | | 00:46.84 |
| 111th | Sabdi Pulido | Denver South (Denver, CO) | Final | | 00:47.57 |
| 112th | Breanna Gonzales | Denver North (Denver, CO) | Final | | 00:47.75 |
| 113th | Jazmin Marquez | Denver North (Denver, CO) | Final | | 00:48.59 |
| 114th | Nataly Miranda | Thomas Jefferson (Denver, CO) | Final | | 00:49.34 |
| 115th | Eva Severance | Denver North (Denver, CO) | Final | | 00:49.41 |
| 116th | Flor Camarena | Lincoln (Denver, CO) | Final | | 00:50.14 |
| 117th | Isabella Sequera | Thomas Jefferson (Denver, CO) | Final | | 00:51.94 |
| 118th | Xyla Ontiveros | Northfield (Denver, CO) | Final | | 00:55.25 |
| 119th | Karina Luna | Lincoln (Denver, CO) | Final | | 00:58.57 |
| 120th | Gabrielle Gomez | Denver North (Denver, CO) | Final | | 01:10.00 |
