| 1st | Anna Shaw | Heritage (Littleton, CO) | Final | | 00:52.81 |
| 2nd | Anna Shaw | Heritage (Littleton, CO) | Preliminary | | 00:54.05 |
| 3rd | Courtney Linton | Legend (Parker, CO) | Preliminary | | 00:54.70 |
| 4th | Courtney Linton | Legend (Parker, CO) | Final | | 00:55.19 |
| 5th | Emily Kahn | George Washington (Denver, CO) | Final | | 00:55.25 |
| 6th | Lindsey Sowitch | Mullen (Denver, CO) | Final | | 00:55.55 |
| 7th | Gabi Roehr | Liberty (Colorado Springs, CO) | Preliminary | | 00:56.07 |
| 8th | Gabi Roehr | Liberty (Colorado Springs, CO) | Final | | 00:56.22 |
| 9th | Emily Kahn | George Washington (Denver, CO) | Preliminary | | 00:56.41 |
| 10th | Katie Power | Smoky Hill (Aurora, CO) | Final | | 00:56.48 |
| 11th | Lindsey Sowitch | Mullen (Denver, CO) | Preliminary | | 00:56.71 |
| 12th | Katie Power | Smoky Hill (Aurora, CO) | Preliminary | | 00:56.77 |
| 13th | Claire Neid | George Washington (Denver, CO) | Preliminary | | 00:56.93 |
| 14th | Kali Stier | Chatfield (Littleton, CO) | Final | | 00:57.02 |
| 15th | Sofia Zinis | Mullen (Denver, CO) | Final | | 00:57.47 |
| 16th | Ellison Bonds | Legend (Parker, CO) | Preliminary | | 00:57.73 |
| 17th | Kali Stier | Chatfield (Littleton, CO) | Preliminary | | 00:57.95 |
| 18th | Sofia Zinis | Mullen (Denver, CO) | Preliminary | | 00:58.01 |
| 19th | Kimbery Nguyen | Eaglecrest (Centennial, CO) | Final | | 00:58.11 |
| 20th | Alyssa Lopez | Heritage (Littleton, CO) | Preliminary | | 00:58.11 |
| 21st | Taylor Nielsen | Mullen (Denver, CO) | Final | | 00:58.15 |
| 22nd | Alyssa Lopez | Heritage (Littleton, CO) | Final | | 00:58.18 |
| 23rd | Taylor Nielsen | Mullen (Denver, CO) | Preliminary | | 00:58.19 |
| 24th | Claire Neid | George Washington (Denver, CO) | Final | | 00:58.26 |
| 25th | Casey Milliman | Heritage (Littleton, CO) | Final | | 00:58.37 |
| 26th | Kaity Brookes | Cherokee Trail (Aurora, CO) | Preliminary | | 00:58.56 |
| 27th | Kaity Brookes | Cherokee Trail (Aurora, CO) | Final | | 00:58.71 |
| 28th | Kimbery Nguyen | Eaglecrest (Centennial, CO) | Preliminary | | 00:58.76 |
| 29th | Casey Milliman | Heritage (Littleton, CO) | Preliminary | | 00:58.80 |
| 30th | Maddie Chapman | Cherokee Trail (Aurora, CO) | Final | | 00:59.05 |
| 31st | Ellison Bonds | Legend (Parker, CO) | Final | | 00:59.06 |
| 32nd | Grace McCrary | Liberty (Colorado Springs, CO) | Preliminary | | 00:59.15 |
| 33rd | Jenna Kondracki | Liberty (Colorado Springs, CO) | Preliminary | | 00:59.30 |
| 34th | Maddie Chapman | Cherokee Trail (Aurora, CO) | Preliminary | | 00:59.31 |
| 35th | Sarah Gaines | Liberty (Colorado Springs, CO) | Preliminary | | 00:59.33 |
| 36th | Grace McCrary | Liberty (Colorado Springs, CO) | Final | | 00:59.34 |
| 37th | Jenna Kondracki | Liberty (Colorado Springs, CO) | Final | | 00:59.65 |
| 38th | Gabrielle Zinis | Mullen (Denver, CO) | Preliminary | | 00:59.74 |
| 39th | Jules Glist | Chatfield (Littleton, CO) | Preliminary | | 00:59.96 |
| 40th | Meghan Mai | Cherokee Trail (Aurora, CO) | Preliminary | | 01:00.00 |
| 41st | Kendall Smith-Williams | Chatfield (Littleton, CO) | Preliminary | | 01:00.22 |
| 42nd | Sarah Beelaert | Chatfield (Littleton, CO) | Preliminary | | 01:00.26 |
| 43rd | Lauryn Crabtree | Douglas County (Castle Rock, CO) | Preliminary | | 01:00.43 |
| 44th | Danielle Azralon | Legend (Parker, CO) | Preliminary | | 01:00.78 |
| 45th | Ella Stasiak | Douglas County (Castle Rock, CO) | Preliminary | | 01:01.44 |
| 46th | Abby Ulrich | Chaparral (Parker, CO) | Preliminary | | 01:01.69 |
| 47th | Isabel Tseng | George Washington (Denver, CO) | Preliminary | | 01:01.85 |
| 48th | Sydney Stewart | Chaparral (Parker, CO) | Preliminary | | 01:01.93 |
| 49th | Abigail Croasdell | Douglas County (Castle Rock, CO) | Preliminary | | 01:01.94 |
| 50th | Nicole Rangel | Cherokee Trail (Aurora, CO) | Preliminary | | 01:02.17 |
| 51st | Kendall Bergstrom | Smoky Hill (Aurora, CO) | Preliminary | | 01:02.27 |
| 52nd | Mariah Graves | Douglas County (Castle Rock, CO) | Preliminary | | 01:02.51 |
| 53rd | Ivy Newton | Smoky Hill (Aurora, CO) | Preliminary | | 01:02.71 |
| 54th | Bella Smith | George Washington (Denver, CO) | Preliminary | | 01:03.02 |
| 55th | Emily Milla | Legend (Parker, CO) | Preliminary | | 01:03.21 |
| 56th | Danielle Ekstrand | Denver South (Denver, CO) | Preliminary | | 01:03.33 |
| 57th | Jolie Yates | Chaparral (Parker, CO) | Preliminary | | 01:03.56 |
| 58th | Katlynn Stephens | Ponderosa (Parker, CO) | Preliminary | | 01:03.78 |
| 59th | Lucy Forrest | Chaparral (Parker, CO) | Preliminary | | 01:04.40 |
| 60th | Morgan Westby | Ponderosa (Parker, CO) | Preliminary | | 01:07.89 |
| 61st | Sydney Miller | Smoky Hill (Aurora, CO) | Preliminary | | 01:09.51 |
| 62nd | Tillie Pinkowitz | Denver South (Denver, CO) | Preliminary | | 01:10.16 |
| 63rd | Jasmine Browning | Eaglecrest (Centennial, CO) | Preliminary | | 01:10.54 |
| 64th | Kelsey McClanahan | Eaglecrest (Centennial, CO) | Preliminary | | 01:10.88 |
| 65th | Alejandra Selenke | Eaglecrest (Centennial, CO) | Preliminary | | 01:12.82 |
| 66th | Zoe Malone | Denver South (Denver, CO) | Preliminary | | 01:13.56 |
| 67th | Maya Baltz | Ponderosa (Parker, CO) | Preliminary | | 01:14.48 |
| 68th | Anna Ward | Ponderosa (Parker, CO) | Preliminary | | 01:15.80 |
| 69th | Anne Welch | Denver South (Denver, CO) | Preliminary | | 01:16.04 |
