| 1st | Josie Fields | Denver East (Denver, CO) | Final | | 00:26.84 |
| 2nd | Kerith Reilly | Denver East (Denver, CO) | Final | | 00:27.55 |
| 3rd | Annika Abramson | George Washington (Denver, CO) | Final | | 00:27.69 |
| 4th | Emma Gentry | Denver North (Denver, CO) | Final | | 00:27.70 |
| 5th | Grace Lyford | Denver East (Denver, CO) | Final | | 00:27.81 |
| 6th | Rowan Hillhouse | Denver East (Denver, CO) | Final | | 00:27.87 |
| 7th | Lila Brimah | Denver East (Denver, CO) | Final | | 00:27.88 |
| 8th | Gabriella Robinson | Denver East (Denver, CO) | Final | | 00:27.98 |
| 9th | Beatrix Kelly | George Washington (Denver, CO) | Final | | 00:28.21 |
| 10th | Elliot Rogers | Denver East (Denver, CO) | Final | | 00:28.30 |
| 11th | Abilgail Slater | Denver South (Denver, CO) | Final | | 00:28.57 |
| 12th | Ella Terch | Denver East (Denver, CO) | Final | | 00:28.59 |
| 13th | Gabrielle Densen | Denver East (Denver, CO) | Final | | 00:28.68 |
| 14th | Jenna Bryant | George Washington (Denver, CO) | Final | | 00:28.80 |
| 15th | Louisa Fickes | George Washington (Denver, CO) | Final | | 00:29.46 |
| 16th | Elizabeth Heitiman | Denver South (Denver, CO) | Final | | 00:29.52 |
| 17th | Bailey Nickols | Denver East (Denver, CO) | Final | | 00:29.58 |
| 18th | Georgia Lamoreaux | Denver East (Denver, CO) | Final | | 00:29.61 |
| 19th | Charlize Briggs | Northfield (Denver, CO) | Final | | 00:29.78 |
| 20th | Kate McVey | Denver East (Denver, CO) | Final | | 00:30.06 |
| 21st | Ella Eichberg | Denver East (Denver, CO) | Final | | 00:30.22 |
| 22nd | Anna Winnecke | Denver East (Denver, CO) | Final | | 00:30.23 |
| 23rd | Sophie Messer | Denver North (Denver, CO) | Final | | 00:30.24 |
| 24th | Sonya Hay | Denver East (Denver, CO) | Final | | 00:30.31 |
| 25th | Katherine Barker | Denver East (Denver, CO) | Final | | 00:30.34 |
| 26th | Lenora DeVoe | Denver East (Denver, CO) | Final | | 00:30.48 |
| 27th | Gabriella DeMaria | Thomas Jefferson (Denver, CO) | Final | | 00:30.52 |
| 28th | Anna Wu | Northfield (Denver, CO) | Final | | 00:30.64 |
| 29th | Georgia Hartley | Thomas Jefferson (Denver, CO) | Final | | 00:30.67 |
| 30th | Avery LoJacono | Thomas Jefferson (Denver, CO) | Final | | 00:31.10 |
| 31st | Katheryn Robinson | Denver North (Denver, CO) | Final | | 00:31.14 |
| 32nd | Grace McClung | Denver South (Denver, CO) | Final | | 00:31.28 |
| 33rd | Alex Stone | Denver North (Denver, CO) | Final | | 00:31.40 |
| 34th | Katharine Olson | Northfield (Denver, CO) | Final | | 00:31.60 |
| 35th | Piper Ahern | Denver North (Denver, CO) | Final | | 00:31.61 |
| 36th | Kate Adams | Denver North (Denver, CO) | Final | | 00:31.77 |
| 37th | Zoe Gerbig | Denver North (Denver, CO) | Final | | 00:31.94 |
| 38th | Peren Yilmazturk | Denver East (Denver, CO) | Final | | 00:32.09 |
| 39th | Elsa Manly | Denver East (Denver, CO) | Final | | 00:32.26 |
| 40th | Addison Whittington | Northfield (Denver, CO) | Final | | 00:32.48 |
| 41st | Georgia Lenhardt | Denver East (Denver, CO) | Final | | 00:32.64 |
| 42nd | Fiona Cahill | Northfield (Denver, CO) | Final | | 00:32.68 |
| 43rd | Rodayna Eissa | Northfield (Denver, CO) | Final | | 00:32.88 |
| 44th | Haley Ekstrand | Denver South (Denver, CO) | Final | | 00:32.91 |
| 45th | Abigail Cunningham | Denver South (Denver, CO) | Final | | 00:32.96 |
| 46th | Amelia Carson | Northfield (Denver, CO) | Final | | 00:32.98 |
| 47th | Jasmin Fischer | Northfield (Denver, CO) | Final | | 00:33.00 |
| 48th | Hallie Lowe | George Washington (Denver, CO) | Final | | 00:33.05 |
| 49th | Emerson Althaus | Thomas Jefferson (Denver, CO) | Final | | 00:33.08 |
| 50th | Sophie Lieb | Northfield (Denver, CO) | Final | | 00:33.09 |
| 51st | DeeAnna Conrrado | Northfield (Denver, CO) | Final | | 00:33.14 |
| 52nd | Brooklin Stromberg | Northfield (Denver, CO) | Final | | 00:33.19 |
| 53rd | Caitlyn Aguilar | Denver South (Denver, CO) | Final | | 00:33.21 |
| 54th | Riley Minogue-Rau | George Washington (Denver, CO) | Final | | 00:33.30 |
| 55th | Sophia Fabian | George Washington (Denver, CO) | Final | | 00:33.50 |
| 56th | Maddie Hensel | Denver North (Denver, CO) | Final | | 00:33.68 |
| 57th | Isabella Medina | Denver South (Denver, CO) | Final | | 00:33.74 |
| 58th | Lauren Leonhardt | Northfield (Denver, CO) | Final | | 00:33.77 |
| 59th | Halle Guber | George Washington (Denver, CO) | Final | | 00:33.82 |
| 60th | Ava Oliva | Northfield (Denver, CO) | Final | | 00:33.82 |
| 61st | Yamilet Gonzalez | Denver South (Denver, CO) | Final | | 00:33.86 |
| 62nd | Emma Khadem | Denver East (Denver, CO) | Final | | 00:34.25 |
| 63rd | Anna Hay | Denver East (Denver, CO) | Final | | 00:34.39 |
| 64th | Reagan Lockwood | Northfield (Denver, CO) | Final | | 00:34.44 |
| 65th | Sofia Weinberg | Denver South (Denver, CO) | Final | | 00:34.62 |
| 66th | Vanessa Roberts | Denver East (Denver, CO) | Final | | 00:34.62 |
| 67th | Aftyn Dewhurst | Denver North (Denver, CO) | Final | | 00:34.73 |
| 68th | Sawyer Fenton | Denver South (Denver, CO) | Final | | 00:34.80 |
| 69th | Lily Koehler | Denver North (Denver, CO) | Final | | 00:34.88 |
| 70th | Ariel Overholt | Northfield (Denver, CO) | Final | | 00:34.94 |
| 71st | Emerson Whitford | George Washington (Denver, CO) | Final | | 00:34.97 |
| 72nd | Mia Ferrero-Lampron | George Washington (Denver, CO) | Final | | 00:35.04 |
| 73rd | Lucy Caldwell | Denver North (Denver, CO) | Final | | 00:35.14 |
| 74th | Zoe Kerr | Denver North (Denver, CO) | Final | | 00:35.19 |
| 75th | Izzy "Puff" Bateman | Denver North (Denver, CO) | Final | | 00:35.20 |
| 76th | Natalie Mawson | George Washington (Denver, CO) | Final | | 00:35.65 |
| 77th | Susanna Imhof | Northfield (Denver, CO) | Final | | 00:35.66 |
| 78th | Jordyn Broughton | Denver North (Denver, CO) | Final | | 00:35.69 |
| 79th | Devon Seagle | Denver North (Denver, CO) | Final | | 00:35.81 |
| 80th | Celia Gauerke | Denver North (Denver, CO) | Final | | 00:35.92 |
| 81st | Ellie Arnquist | Northfield (Denver, CO) | Final | | 00:36.33 |
| 82nd | Norah Cohen | George Washington (Denver, CO) | Final | | 00:36.48 |
| 83rd | Jayla Casados | Thomas Jefferson (Denver, CO) | Final | | 00:36.52 |
| 84th | Stella Ziegenhagen | Thomas Jefferson (Denver, CO) | Final | | 00:36.62 |
| 85th | Claire Crimmins | Denver East (Denver, CO) | Final | | 00:36.90 |
| 86th | Teresa Silveira | Thomas Jefferson (Denver, CO) | Final | | 00:36.98 |
| 87th | Grace Davis | George Washington (Denver, CO) | Final | | 00:37.36 |
| 88th | Elanor Konosky | Denver South (Denver, CO) | Final | | 00:37.84 |
| 89th | Audrey Hermes | Northfield (Denver, CO) | Final | | 00:37.89 |
| 90th | Anna DelPonte | Thomas Jefferson (Denver, CO) | Final | | 00:37.96 |
| 91st | Evelyn Ferguson | George Washington (Denver, CO) | Final | | 00:37.99 |
| 92nd | Madison Hall | Northfield (Denver, CO) | Final | | 00:38.18 |
| 93rd | Payton Eidsness | Northfield (Denver, CO) | Final | | 00:38.57 |
| 94th | Noelle Swartzendruber | Northfield (Denver, CO) | Final | | 00:38.59 |
| 95th | Klara Kiesel | Denver North (Denver, CO) | Final | | 00:38.85 |
| 96th | Alicia Leyba-Valdez | Denver North (Denver, CO) | Final | | 00:38.90 |
| 97th | Catherine Lara | Northfield (Denver, CO) | Final | | 00:38.97 |
| 98th | Violet Eleson | George Washington (Denver, CO) | Final | | 00:39.07 |
| 99th | Kaleigh Johnston | Denver South (Denver, CO) | Final | | 00:39.12 |
| 100th | Xitlali Najar | Denver North (Denver, CO) | Final | | 00:39.67 |
| 101st | Marissa Mohabir | Denver South (Denver, CO) | Final | | 00:40.51 |
| 102nd | Keira Miller | George Washington (Denver, CO) | Final | | 00:40.55 |
| 103rd | Hannah Deixler | Northfield (Denver, CO) | Final | | 00:40.70 |
| 104th | Victoria Arellano | George Washington (Denver, CO) | Final | | 00:40.80 |
| 105th | Adriana Ruiz-Sanz | Northfield (Denver, CO) | Final | | 00:41.00 |
| 106th | Josephine Maes | George Washington (Denver, CO) | Final | | 00:41.31 |
| 107th | Amelie Miller | Thomas Jefferson (Denver, CO) | Final | | 00:41.62 |
| 108th | Samantha Riggleman | George Washington (Denver, CO) | Final | | 00:41.97 |
| 109th | Caroline Brown | Denver South (Denver, CO) | Final | | 00:42.15 |
| 110th | Meredith Lewis | Northfield (Denver, CO) | Final | | 00:42.52 |
| 111th | Camden Campbell | Northfield (Denver, CO) | Final | | 00:42.82 |
| 112th | Eliana Leyba-Valdez | Denver North (Denver, CO) | Final | | 00:43.53 |
| 113th | Dayliha Martinez | Denver North (Denver, CO) | Final | | 00:45.50 |
| 114th | Sadie MAE Mosley | Denver North (Denver, CO) | Final | | 00:45.74 |
| 115th | Caroline Christian-Powell | Northfield (Denver, CO) | Final | | 00:46.41 |
| 116th | Ainsley Bergstrand | Northfield (Denver, CO) | Final | | 00:46.54 |
| 117th | Aislyn Larson | George Washington (Denver, CO) | Final | | 00:46.76 |
| 118th | Macy Whistler | Thomas Jefferson (Denver, CO) | Final | | 00:47.29 |
| 119th | Autumn Cross | Denver South (Denver, CO) | Final | | 00:48.34 |
| 120th | Nichole Saucedo | Northfield (Denver, CO) | Final | | 00:48.96 |
| 121st | Lou Tujague | Denver South (Denver, CO) | Final | | 00:49.38 |
| 122nd | Kaylee McDermott | Denver South (Denver, CO) | Final | | 00:52.80 |
| 123rd | Adison Towles | Northfield (Denver, CO) | Final | | 00:55.10 |
| 124th | Paula Acosta | George Washington (Denver, CO) | Final | | 01:07.66 |
| 125th | Silvia Cruz | Lincoln (Denver, CO) | Final | | 01:08.82 |
| 126th | Astrid Miranda | Lincoln (Denver, CO) | Final | | 01:26.92 |
| 127th | Juliana Colorado | Lincoln (Denver, CO) | Final | | 01:27.64 |
| 128th | Daniella Romero | Lincoln (Denver, CO) | Final | | 01:57.85 |
