| 1st | Abigail Perry | Lewis-Palmer (Monument, CO) | Final | | 00:23.95 |
| 2nd | Abigail Perry | Lewis-Palmer (Monument, CO) | Preliminary | | 00:24.43 |
| 3rd | Julia Thomas | Discovery Canyon (Colorado Springs, CO) | Final | | 00:24.62 |
| 4th | Julia Thomas | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:24.99 |
| 5th | Emily Poe | Cheyenne Mountain (Colorado Springs, CO) | Final | | 00:25.32 |
| 6th | Alex Lombardi | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:25.61 |
| 7th | Emily Poe | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:25.71 |
| 8th | Alex Lombardi | Cheyenne Mountain (Colorado Springs, CO) | Final | | 00:25.72 |
| 9th | Lilly Pike | Lewis-Palmer (Monument, CO) | Final | | 00:26.42 |
| 10th | Miette Alberts | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:26.55 |
| 11th | Kaitlyn Vigil | Air Academy (US Air Force Academy, CO) | Final | | 00:26.68 |
| 12th | Lilly Pike | Lewis-Palmer (Monument, CO) | Preliminary | | 00:26.70 |
| 13th | Nicola Ferrante | Lewis-Palmer (Monument, CO) | Final | | 00:26.73 |
| 14th | Nicola Ferrante | Lewis-Palmer (Monument, CO) | Preliminary | | 00:26.74 |
| 15th | Miette Alberts | Cheyenne Mountain (Colorado Springs, CO) | Final | | 00:26.81 |
| 16th | Ashlyn DeCaires | Lewis-Palmer (Monument, CO) | Preliminary | | 00:27.00 |
| 17th | Ashlyn DeCaires | Lewis-Palmer (Monument, CO) | Final | | 00:27.07 |
| 18th | Aubrie Iverson | Air Academy (US Air Force Academy, CO) | Final | | 00:27.53 |
| 19th | Maggie Lacuzong | Cheyenne Mountain (Colorado Springs, CO) | Final | | 00:27.71 |
| 20th | Michaela Edelbach | Lewis-Palmer (Monument, CO) | Preliminary | | 00:27.74 |
| 21st | Kaitlyn Vigil | Air Academy (US Air Force Academy, CO) | Preliminary | | 00:27.79 |
| 22nd | Maggie Lacuzong | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:27.94 |
| 23rd | Aubrie Iverson | Air Academy (US Air Force Academy, CO) | Preliminary | | 00:27.96 |
| 24th | Sarah Zetocha | Lewis-Palmer (Monument, CO) | Preliminary | | 00:28.20 |
| 25th | Molly Pattillo | Lewis-Palmer (Monument, CO) | Preliminary | | 00:28.48 |
| 26th | Kennedy Evers | Air Academy (US Air Force Academy, CO) | Preliminary | | 00:28.56 |
| 27th | Braelyn Marr | Lewis-Palmer (Monument, CO) | Preliminary | | 00:28.65 |
| 28th | Kennedy Evers | Air Academy (US Air Force Academy, CO) | Final | | 00:28.65 |
| 29th | Meredith Makowsky | Lewis-Palmer (Monument, CO) | Preliminary | | 00:28.71 |
| 30th | Haley Kofford | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:28.96 |
| 31st | Lilli Marjon | Lewis-Palmer (Monument, CO) | Preliminary | | 00:29.28 |
| 32nd | Kytelyn Pieper | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:29.34 |
| 33rd | Andrea Falt | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:29.44 |
| 34th | Ashleigh Smith | Discovery Canyon (Colorado Springs, CO) | Final | | 00:29.47 |
| 35th | Aryeh Alberts | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:29.49 |
| 36th | Caitlin Beverly | Lewis-Palmer (Monument, CO) | Preliminary | | 00:30.38 |
| 37th | Ashleigh Smith | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:30.57 |
| 38th | Lauren McDowell | Discovery Canyon (Colorado Springs, CO) | Final | | 00:30.77 |
| 39th | Molly Jamison | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:31.44 |
| 40th | Ella Johnson | Lewis-Palmer (Monument, CO) | Preliminary | | 00:32.71 |
| 41st | Lauren McDowell | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:32.75 |
| 42nd | Elena Torres | Lewis-Palmer (Monument, CO) | Preliminary | | 00:32.88 |
| 43rd | July Sterin | Lewis-Palmer (Monument, CO) | Preliminary | | 00:33.02 |
| 44th | Adah McCarley | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:33.03 |
| 45th | Diya Suri | Lewis-Palmer (Monument, CO) | Preliminary | | 00:33.03 |
| 46th | Danica Bishop | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:34.11 |
| 47th | Grace Lopez | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:34.12 |
| 48th | Danica Bishop | Discovery Canyon (Colorado Springs, CO) | Final | | 00:34.12 |
| 49th | Callie Shaw | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:34.48 |
| 50th | Megan Walsh | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:35.99 |
| 51st | Abbey McCown | Air Academy (US Air Force Academy, CO) | Final | | 00:36.65 |
| 52nd | Ashlee Black | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:36.86 |
| 53rd | Jenna Wachlin | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:36.87 |
| 54th | Abbey McCown | Air Academy (US Air Force Academy, CO) | Preliminary | | 00:37.05 |
| 55th | Courtney Beckley | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:37.50 |
| 56th | Kaitlyn Cisneros | Air Academy (US Air Force Academy, CO) | Preliminary | | 00:37.65 |
| 57th | Eileen Wolf | Air Academy (US Air Force Academy, CO) | Preliminary | | 00:38.16 |
| 58th | Melanie Burnett | Air Academy (US Air Force Academy, CO) | Preliminary | | 00:38.39 |
| 59th | Jamie Pyrtle | Air Academy (US Air Force Academy, CO) | Preliminary | | 00:40.72 |
| 60th | Nicole Johnson | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:41.06 |
| 61st | Petrauliana Swanson | Air Academy (US Air Force Academy, CO) | Preliminary | | 00:41.32 |
| 62nd | Misk Aboughali | Cheyenne Mountain (Colorado Springs, CO) | Preliminary | | 00:41.47 |
| 63rd | Victoria Arbizzani | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 00:42.62 |
| 64th | Eva Cincotta | Discovery Canyon (Colorado Springs, CO) | Preliminary | | 02:08.08 |
