| 1st | Maggie Germonprez | Omaha Central (Omaha, NE) | Final | | 00:26.68 |
| 2nd | Makenna Laher | Burke (Omaha, NE) | Final | | 00:26.94 |
| 3rd | Megan Keller | Burke (Omaha, NE) | Final | | 00:27.50 |
| 4th | Emma Nieland | Omaha Central (Omaha, NE) | Final | | 00:27.74 |
| 5th | Ella Frisbie | Burke (Omaha, NE) | Final | | 00:28.17 |
| 6th | Kylie Westphal | Omaha Central (Omaha, NE) | Final | | 00:28.50 |
| 7th | Grace Gundersen | Burke (Omaha, NE) | Final | | 00:28.52 |
| 8th | Maggie Kramer | Skutt Catholic (Omaha, NE) | Final | | 00:28.72 |
| 9th | Allie Matukewicz | Skutt Catholic (Omaha, NE) | Final | | 00:29.05 |
| 10th | Calen Crockett | Burke (Omaha, NE) | Final | | 00:29.37 |
| 11th | Lauren Anderson | Omaha Central (Omaha, NE) | Final | | 00:29.41 |
| 12th | Chloe Jones | Omaha Central (Omaha, NE) | Final | | 00:29.64 |
| 13th | Kaity Jenkins | Burke (Omaha, NE) | Final | | 00:29.75 |
| 14th | Lucy Parizek | Burke (Omaha, NE) | Final | | 00:29.97 |
| 15th | Katie Evans | Millard West (Omaha, NE) | Final | | 00:30.59 |
| 16th | Bridget Connor | Skutt Catholic (Omaha, NE) | Final | | 00:30.76 |
| 17th | Alison Doody | Omaha Central (Omaha, NE) | Final | | 00:31.17 |
| 18th | Shay Macafee | Omaha Central (Omaha, NE) | Final | | 00:31.26 |
| 19th | Nyeruon Tip | Millard North (Omaha, NE) | Final | | 00:31.43 |
| 20th | Victoria Strubbe | Burke (Omaha, NE) | Final | | 00:31.44 |
| 21st | Kallie Baker | Omaha Central (Omaha, NE) | Final | | 00:31.69 |
| 22nd | Leila Klare | Omaha Central (Omaha, NE) | Final | | 00:31.93 |
| 23rd | Riley Sanden | Omaha Central (Omaha, NE) | Final | | 00:32.07 |
| 24th | Natalie Barclay | Skutt Catholic (Omaha, NE) | Final | | 00:32.12 |
| 25th | Morgan Able | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:32.34 |
| 26th | Devlyn Pithan | Omaha Central (Omaha, NE) | Final | | 00:32.47 |
| 27th | Megan Nevins | Skutt Catholic (Omaha, NE) | Final | | 00:32.54 |
| 28th | Kalthleen Kirshenbaum | Burke (Omaha, NE) | Final | | 00:32.56 |
| 29th | Mica Kielian | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:32.80 |
| 30th | McKenna Grau | Omaha Central (Omaha, NE) | Final | | 00:32.86 |
| 31st | Skye Kusler | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:32.95 |
| 32nd | Jaeli Cole | Skutt Catholic (Omaha, NE) | Final | | 00:33.03 |
| 33rd | Panagea Kaan | Omaha Central (Omaha, NE) | Final | | 00:33.14 |
| 34th | Theresa Crowell | Millard North (Omaha, NE) | Final | | 00:33.28 |
| 35th | Katelyn Petersen | Millard North (Omaha, NE) | Final | | 00:33.32 |
| 36th | Brooke Dumont | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:33.38 |
| 37th | Olivia Lott | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:33.57 |
| 38th | Emily Engel | Omaha Central (Omaha, NE) | Final | | 00:33.65 |
| 39th | Grace Danielson | Millard South (Omaha, NE) | Final | | 00:33.81 |
| 40th | Josie Polacek | Skutt Catholic (Omaha, NE) | Final | | 00:33.83 |
| 41st | Katelyn Ryman | Millard West (Omaha, NE) | Final | | 00:33.98 |
| 42nd | Rachel Johnson | Millard North (Omaha, NE) | Final | | 00:34.07 |
| 43rd | Kadence Christiansen | Millard North (Omaha, NE) | Final | | 00:34.14 |
| 44th | Taryn Moore | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:34.40 |
| 45th | Olivia Theophilus | Omaha Central (Omaha, NE) | Final | | 00:34.40 |
| 46th | Victoria Risager | Omaha Central (Omaha, NE) | Final | | 00:35.02 |
| 47th | Emilee Foix | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:35.73 |
| 48th | Tami Rojo | Millard North (Omaha, NE) | Final | | 00:35.83 |
| 49th | Rhyan Crall | Millard North (Omaha, NE) | Final | | 00:35.95 |
| 50th | Cassandra LaFramboise | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:36.52 |
| 51st | Stephanie Avila | Millard North (Omaha, NE) | Final | | 00:36.56 |
| 52nd | Reeyan Bhakat | Millard North (Omaha, NE) | Final | | 00:36.69 |
| 53rd | Bailey Emrich | Millard North (Omaha, NE) | Final | | 00:36.70 |
| 54th | Christina Cardoza | Millard South (Omaha, NE) | Final | | 00:37.64 |
| 55th | Sydney Peterson | Millard North (Omaha, NE) | Final | | 00:37.66 |
| 56th | Brianna Bayliss | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:37.99 |
| 57th | Lyndsay Vetick | Millard West (Omaha, NE) | Final | | 00:38.06 |
| 58th | Johnna Sisneros | Millard South (Omaha, NE) | Final | | 00:38.10 |
| 59th | Kimberly Fernandez | Millard South (Omaha, NE) | Final | | 00:38.42 |
| 60th | Nicole Kalhorn | Millard South (Omaha, NE) | Final | | 00:38.81 |
| 61st | Kylee Ellison | Millard South (Omaha, NE) | Final | | 00:38.98 |
| 62nd | Ryley Waldschmidt | Millard South (Omaha, NE) | Final | | 00:39.20 |
| 63rd | Kristen McAllister | Millard West (Omaha, NE) | Final | | 00:39.57 |
| 64th | Sheri Poehling | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:39.72 |
| 65th | Mckenzie Duval | Millard West (Omaha, NE) | Final | | 00:39.83 |
| 66th | Alix Kahler | Millard West (Omaha, NE) | Final | | 00:40.02 |
| 67th | Breanna Johnson | Millard South (Omaha, NE) | Final | | 00:40.10 |
| 68th | Cassandra Martin | Millard North (Omaha, NE) | Final | | 00:40.42 |
| 69th | Shreeya Mishra | Millard West (Omaha, NE) | Final | | 00:40.99 |
| 70th | Elizabeth Nelson | Millard North (Omaha, NE) | Final | | 00:41.55 |
| 71st | Chelsea Nderu | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:41.89 |
| 72nd | Rachel Larson | Papillion-LaVista/Papillion-LaVista South (Papillion, NE) | Final | | 00:46.87 |
