| 1st | Lexie Malazdrewicz | Evergreen (CO) | Preliminary | | 00:24.37 |
| 2nd | Colleen Olson | D'Evelyn (Denver, CO) | Preliminary | | 00:24.76 |
| 3rd | Meghan Lahr | Lakewood (CO) | Preliminary | | 00:25.24 |
| 4th | Bailey Smith | Evergreen (CO) | Preliminary | | 00:25.40 |
| 5th | Kelsey Foster | Dakota Ridge (Littleton, CO) | Preliminary | | 00:25.90 |
| 6th | Emily Murphy | Chatfield (Littleton, CO) | Preliminary | | 00:25.93 |
| 7th | Alexa Ainlay | Ralston Valley (Arvada, CO) | Preliminary | | 00:26.04 |
| 8th | Janie Anderson | Green Mountain (Lakewood, CO) | Preliminary | | 00:26.38 |
| 9th | Katherine Nester | Dakota Ridge (Littleton, CO) | Preliminary | | 00:26.49 |
| 10th | Kathryn Cheatum | Golden (CO) | Preliminary | | 00:26.51 |
| 11th | Erin Lemberger | Columbine (Littleton, CO) | Preliminary | | 00:26.59 |
| 12th | Annie Fox | Golden (CO) | Preliminary | | 00:26.59 |
| 13th | Inez Moore | D'Evelyn (Denver, CO) | Preliminary | | 00:26.61 |
| 14th | Kaila Munday | Dakota Ridge (Littleton, CO) | Preliminary | | 00:26.85 |
| 15th | Deseray Sileo | Dakota Ridge (Littleton, CO) | Preliminary | | 00:26.90 |
| 16th | Hailey Gerze | Chatfield (Littleton, CO) | Preliminary | | 00:27.03 |
| 17th | Maggie Burns | Columbine (Littleton, CO) | Preliminary | | 00:27.20 |
| 18th | Caitlyn Kissee | Ralston Valley (Arvada, CO) | Preliminary | | 00:27.30 |
| 19th | Mackenzie Bailey | Lakewood (CO) | Preliminary | | 00:27.40 |
| 20th | Adriana Romeo | Wheat Ridge (CO) | Preliminary | | 00:27.44 |
| 21st | Brooke Bower | Columbine (Littleton, CO) | Preliminary | | 00:27.47 |
| 22nd | Juliana Lucena | Lakewood (CO) | Preliminary | | 00:27.49 |
| 23rd | Colleen Shehan | D'Evelyn (Denver, CO) | Preliminary | | 00:27.56 |
| 24th | Marie Newkirk | Evergreen (CO) | Preliminary | | 00:27.60 |
| 25th | Jessica Urban | Conifer (CO) | Preliminary | | 00:27.68 |
| 26th | Lindsay Gettel | Ralston Valley (Arvada, CO) | Preliminary | | 00:27.69 |
| 27th | Molly Walsh | Lakewood (CO) | Preliminary | | 00:27.71 |
| 28th | Madison Hector | Arvada West (Arvada, CO) | Preliminary | | 00:27.82 |
| 29th | Amy Cazier | Wheat Ridge (CO) | Preliminary | | 00:28.12 |
| 30th | Katherine Sims | Chatfield (Littleton, CO) | Preliminary | | 00:28.15 |
| 31st | Allison Perkins | Chatfield (Littleton, CO) | Preliminary | | 00:28.28 |
| 32nd | Lori Lefevre | D'Evelyn (Denver, CO) | Preliminary | | 00:28.63 |
| 33rd | Brenna Kroker | Standley Lake (Westminster, CO) | Preliminary | | 00:28.85 |
| 34th | Daria Romero | Bear Creek (Lakewood, CO) | Preliminary | | 00:28.92 |
| 35th | Shayna Kurtz | Pomona (Arvada, CO) | Preliminary | | 00:29.02 |
| 36th | Kayla Wolfe | Pomona (Arvada, CO) | Preliminary | | 00:29.18 |
| 37th | Anisia Barnett | Evergreen (CO) | Preliminary | | 00:29.24 |
| 38th | Hanna Long | Green Mountain (Lakewood, CO) | Preliminary | | 00:29.30 |
| 39th | Mia Lamirand | Arvada (CO) | Preliminary | | 00:29.41 |
| 40th | Marguerite Buchmann | Arvada West (Arvada, CO) | Preliminary | | 00:29.62 |
| 41st | Shelly Browne | Wheat Ridge (CO) | Preliminary | | 00:29.82 |
| 42nd | Brenna Conway | Wheat Ridge (CO) | Preliminary | | 00:29.90 |
| 43rd | Morgan Wagner | Ralston Valley (Arvada, CO) | Preliminary | | 00:30.03 |
| 44th | Elaine Wyatt | Green Mountain (Lakewood, CO) | Preliminary | | 00:30.06 |
| 45th | Jordyn Komoras | Golden (CO) | Preliminary | | 00:30.17 |
| 46th | Gabby Lopez | Columbine (Littleton, CO) | Preliminary | | 00:30.32 |
| 47th | Donalyn Bliss | Arvada West (Arvada, CO) | Preliminary | | 00:30.40 |
| 48th | Taylor Landin | Green Mountain (Lakewood, CO) | Preliminary | | 00:30.92 |
| 49th | Sarah Jones | Arvada (CO) | Preliminary | | 00:31.38 |
| 50th | Stephanie Pascua | Pomona (Arvada, CO) | Preliminary | | 00:31.44 |
| 51st | Jacquelyn Seifert | Conifer (CO) | Preliminary | | 00:31.47 |
| 52nd | Kaitlin Shulter | Arvada (CO) | Preliminary | | 00:31.61 |
| 53rd | Mallory Tjaden | Conifer (CO) | Preliminary | | 00:31.68 |
| 54th | Brenna Fowler | Standley Lake (Westminster, CO) | Preliminary | | 00:31.73 |
| 55th | Brenna LeBlanc | Conifer (CO) | Preliminary | | 00:31.82 |
| 56th | Kristen Romano | Bear Creek (Lakewood, CO) | Preliminary | | 00:32.16 |
| 57th | Samantha Thorfinson | Standley Lake (Westminster, CO) | Preliminary | | 00:32.58 |
| 58th | Taylor McRae | Standley Lake (Westminster, CO) | Preliminary | | 00:32.68 |
| 59th | Ashley Cochran | Arvada West (Arvada, CO) | Preliminary | | 00:32.97 |
| 60th | Julia Baskin | Pomona (Arvada, CO) | Preliminary | | 00:33.79 |
| 61st | Noelle Cohn | Golden (CO) | Preliminary | | 00:35.21 |
| 62nd | Morgan Wunder | Bear Creek (Lakewood, CO) | Preliminary | | 00:35.87 |
| 63rd | Mikaela King | Bear Creek (Lakewood, CO) | Preliminary | | 00:36.20 |
