| 1st | Marina Fonseca | Thompson Valley (Loveland, CO) | Preliminary | | 00:25.49 |
| 2nd | Zoie Jordan | Niwot (CO) | Preliminary | | 00:25.87 |
| 3rd | Kendall Wingfield | Broomfield (CO) | Preliminary | | 00:26.20 |
| 4th | Jennifer Young | Broomfield (CO) | Preliminary | | 00:26.51 |
| 5th | Sara Nienaber | Thompson Valley (Loveland, CO) | Preliminary | | 00:26.55 |
| 6th | Jessie Moore | Niwot (CO) | Preliminary | | 00:26.84 |
| 7th | Alyssa Eidem | Thompson Valley (Loveland, CO) | Preliminary | | 00:26.91 |
| 8th | Madison Studebaker | Niwot (CO) | Preliminary | | 00:26.99 |
| 9th | Sierra Juvonen | Silver Creek (Longmont, CO) | Preliminary | | 00:27.30 |
| 10th | Sydnee King | Thompson Valley (Loveland, CO) | Preliminary | | 00:27.34 |
| 11th | Angela Walter | Silver Creek (Longmont, CO) | Preliminary | | 00:27.52 |
| 12th | Madison Pelton | Silver Creek (Longmont, CO) | Preliminary | | 00:27.65 |
| 13th | Jessica Thompson | Longmont (CO) | Preliminary | | 00:27.68 |
| 14th | Annie Yudnich | Broomfield (CO) | Preliminary | | 00:27.82 |
| 15th | Julie Morrison | Longmont (CO) | Preliminary | | 00:27.86 |
| 16th | Erin Moroze | Broomfield (CO) | Preliminary | | 00:27.93 |
| 17th | Kathryn Trees | Silver Creek (Longmont, CO) | Preliminary | | 00:27.94 |
| 18th | Brianna Utley | Mountain View (Loveland, CO) | Preliminary | | 00:28.16 |
| 19th | Alyson Lhungay | Silver Creek (Longmont, CO) | Preliminary | | 00:28.28 |
| 20th | Hannah Thramann | Niwot (CO) | Preliminary | | 00:28.45 |
| 21st | Savanna Horst | Thompson Valley (Loveland, CO) | Preliminary | | 00:28.50 |
| 22nd | Katie Gillett | Niwot (CO) | Preliminary | | 00:28.56 |
| 23rd | Sofia Ahlberg | Silver Creek (Longmont, CO) | Preliminary | | 00:28.87 |
| 24th | Courtney Dobbins | Silver Creek (Longmont, CO) | Preliminary | | 00:28.90 |
| 25th | Sidney Wach | Silver Creek (Longmont, CO) | Preliminary | | 00:28.97 |
| 26th | Clarissa Davies | Silver Creek (Longmont, CO) | Preliminary | | 00:29.27 |
| 27th | Emma Heichelheim | Silver Creek (Longmont, CO) | Preliminary | | 00:29.53 |
| 28th | Maisey Harrison | Niwot (CO) | Preliminary | | 00:29.73 |
| 29th | Annika Jarvinen | Niwot (CO) | Preliminary | | 00:29.84 |
| 30th | Tyne Curran | Niwot (CO) | Preliminary | | 00:29.99 |
| 31st | Madi Polansky | Mountain View (Loveland, CO) | Preliminary | | 00:30.38 |
| 32nd | Ingrid Zimmerman | Niwot (CO) | Preliminary | | 00:30.49 |
| 33rd | Saleen Chioda | Longmont (CO) | Preliminary | | 00:30.54 |
| 34th | Mindy Jones | Broomfield (CO) | Preliminary | | 00:30.68 |
| 35th | Chloe King | Niwot (CO) | Preliminary | | 00:30.68 |
| 36th | Sierra Milam | Silver Creek (Longmont, CO) | Preliminary | | 00:30.71 |
| 37th | Miranda Sheets | Thompson Valley (Loveland, CO) | Preliminary | | 00:30.74 |
| 38th | Peyton Wright | Niwot (CO) | Preliminary | | 00:30.82 |
| 39th | Amy Lovstedt | Silver Creek (Longmont, CO) | Preliminary | | 00:30.95 |
| 40th | Dana Maydew | Niwot (CO) | Preliminary | | 00:31.03 |
| 41st | Kayly Fox | Silver Creek (Longmont, CO) | Preliminary | | 00:31.08 |
| 42nd | Matalin Horn | Longmont (CO) | Preliminary | | 00:31.21 |
| 43rd | Sonia Merino | Thompson Valley (Loveland, CO) | Preliminary | | 00:31.26 |
| 44th | Laura Widmer | Silver Creek (Longmont, CO) | Preliminary | | 00:31.44 |
| 45th | Courtney Kempf | Niwot (CO) | Preliminary | | 00:31.49 |
| 46th | Kelcy Coleman | Longmont (CO) | Preliminary | | 00:31.56 |
| 47th | Ashley Weibel | Niwot (CO) | Preliminary | | 00:31.81 |
| 48th | Amanda Burns | Silver Creek (Longmont, CO) | Preliminary | | 00:31.82 |
| 49th | Elizabeth Cameron | Longmont (CO) | Preliminary | | 00:31.87 |
| 50th | Elise Jones | Silver Creek (Longmont, CO) | Preliminary | | 00:31.88 |
| 51st | Katelyn Riley | Thompson Valley (Loveland, CO) | Preliminary | | 00:31.95 |
| 52nd | Hannah Foster | Silver Creek (Longmont, CO) | Preliminary | | 00:31.97 |
| 53rd | Meghan White | Thompson Valley (Loveland, CO) | Preliminary | | 00:31.99 |
| 54th | Madeline Peterson | Longmont (CO) | Preliminary | | 00:32.01 |
| 55th | Breeana Dyksra | Thompson Valley (Loveland, CO) | Preliminary | | 00:32.03 |
| 56th | Baylee Diederich | Longmont (CO) | Preliminary | | 00:32.11 |
| 57th | Kristy Lee | Longmont (CO) | Preliminary | | 00:32.14 |
| 58th | Sydney Schwartz | Thompson Valley (Loveland, CO) | Preliminary | | 00:32.31 |
| 59th | Izzy Fischer | Silver Creek (Longmont, CO) | Preliminary | | 00:32.43 |
| 60th | Cathy Gomez | Mountain View (Loveland, CO) | Preliminary | | 00:32.48 |
| 61st | Anna Harvey | Longmont (CO) | Preliminary | | 00:32.48 |
| 62nd | Belen Vidal | Thompson Valley (Loveland, CO) | Preliminary | | 00:32.56 |
| 63rd | Maddy Williams | Broomfield (CO) | Preliminary | | 00:32.71 |
| 64th | Elizabeth Owens | Longmont (CO) | Preliminary | | 00:32.88 |
| 65th | Aspen Pauluhn | Niwot (CO) | Preliminary | | 00:32.95 |
| 66th | Abby Johnson | Longmont (CO) | Preliminary | | 00:33.03 |
| 67th | Dana Dow | Silver Creek (Longmont, CO) | Preliminary | | 00:33.08 |
| 68th | Kiera Pitts | Niwot (CO) | Preliminary | | 00:33.10 |
| 69th | Sapphire Hern | Niwot (CO) | Preliminary | | 00:33.17 |
| 70th | Paige Ebbers | Niwot (CO) | Preliminary | | 00:33.27 |
| 71st | Alexandra Liebman | Thompson Valley (Loveland, CO) | Preliminary | | 00:33.32 |
| 72nd | Elle Bartelson | Mountain View (Loveland, CO) | Preliminary | | 00:33.37 |
| 73rd | Jillian Bauer | Silver Creek (Longmont, CO) | Preliminary | | 00:33.42 |
| 74th | Brianna Wolfe | Longmont (CO) | Preliminary | | 00:33.45 |
| 75th | Katie Green | Longmont (CO) | Preliminary | | 00:33.59 |
| 76th | Hannah Alvarado | Niwot (CO) | Preliminary | | 00:33.62 |
| 77th | Mackenzie Parker | Mountain View (Loveland, CO) | Preliminary | | 00:33.72 |
| 78th | Rachel Blanton | Silver Creek (Longmont, CO) | Preliminary | | 00:33.75 |
| 79th | Sarah Dalke | Broomfield (CO) | Preliminary | | 00:33.81 |
| 80th | Marin Roth | Silver Creek (Longmont, CO) | Preliminary | | 00:34.25 |
| 81st | Allyson Barnhill | Thompson Valley (Loveland, CO) | Preliminary | | 00:34.29 |
| 82nd | Lydia Oliver | Silver Creek (Longmont, CO) | Preliminary | | 00:34.40 |
| 83rd | Vanessa Romero | Thompson Valley (Loveland, CO) | Preliminary | | 00:34.48 |
| 84th | Amanda McCay | Silver Creek (Longmont, CO) | Preliminary | | 00:35.10 |
| 85th | Tori Biggerstaff | Broomfield (CO) | Preliminary | | 00:35.11 |
| 86th | Laura Hanneken | Silver Creek (Longmont, CO) | Preliminary | | 00:35.16 |
| 87th | Sophie Nelson | Silver Creek (Longmont, CO) | Preliminary | | 00:35.74 |
| 88th | Courtney Giles | Broomfield (CO) | Preliminary | | 00:35.96 |
| 89th | Annie Birkeland | Silver Creek (Longmont, CO) | Preliminary | | 00:36.03 |
| 90th | Kourtney Rybicki | Mountain View (Loveland, CO) | Preliminary | | 00:36.22 |
| 91st | Delayne Johnson | Niwot (CO) | Preliminary | | 00:36.33 |
| 92nd | Kaitlyn Woller | Mountain View (Loveland, CO) | Preliminary | | 00:36.59 |
| 93rd | Alicia Marquez | Broomfield (CO) | Preliminary | | 00:36.96 |
| 94th | Maiya Jazwierska | Silver Creek (Longmont, CO) | Preliminary | | 00:37.38 |
| 95th | Larisa Loebe | Thompson Valley (Loveland, CO) | Preliminary | | 00:37.39 |
| 96th | Mariah McEvoy | Mountain View (Loveland, CO) | Preliminary | | 00:39.03 |
| 97th | Matilde Ghe | Silver Creek (Longmont, CO) | Preliminary | | 00:39.13 |
| 98th | Kendall Tarpley | Silver Creek (Longmont, CO) | Preliminary | | 00:39.54 |
| 99th | Madisyn Gerondale | Silver Creek (Longmont, CO) | Preliminary | | 00:39.91 |
| 100th | Brittany Burns | Thompson Valley (Loveland, CO) | Preliminary | | 00:39.98 |
| 101st | Kianna Lauck | Longmont (CO) | Preliminary | | 00:40.00 |
| 102nd | Carrie Millard | Silver Creek (Longmont, CO) | Preliminary | | 00:40.64 |
| 103rd | Dierdre Ouellette | Niwot (CO) | Preliminary | | 00:41.01 |
| 104th | Katelyn Feller | Silver Creek (Longmont, CO) | Preliminary | | 00:41.74 |
| 105th | Lyna Pham | Longmont (CO) | Preliminary | | 00:42.62 |
| 106th | Amberli Redford | Mountain View (Loveland, CO) | Preliminary | | 00:51.99 |
