| 2A District 1 | Higher Seed Host Quarterfinals; Buchholz HS, FL |
| 2A District 2 | Fletcher HS, FL |
| 2A District 3 | Ponte Vedra HS, FL |
| 2A District 4 | Lake Mary HS, FL |
| 2A District 5 | West Orange HS, FL |
| 2A District 6 | Lake Howell High School, FL |
| 2A District 7 | Dr. Phillips HS, FL |
| 2A District 8 | FL |
| 2A District 9 | Mitchell High School, FL |
| 2A District 10 | Plant High School, FL |
| 2A District 11 | Highest Seed, FL |
| 2A District 12 | Highest Seed, FL |
| 2A District 13 | FL |
| 2A District 14 | Higher Seed Host Quarterfinals; Boca Raton HS, FL |
| 2A District 15 | St. Thomas Aquinas, FL |
| 2A District 16 | Columbus H.S., FL |
| 1A District 1 | West Florida High School, FL |
| 1A District 2 | Maclay HS, FL |
| 1A District 3 | Episcopal School, FL |
| 1A District 4 | Pedro Menendez High School, FL |
| 1A District 5 | Lake Highland Prep, FL |
| 1A District 6 | All Saints Academy, FL |
| 1A District 7 | Melbourne Central Catholic, FL |
| 1A District 8 | St. Edwards HS, FL |
| 1A District 9 | Berkely Prep, FL |
| 1A District 10 | High Seed Hosts, FL |
| 1A District 11 | Higher Seed Host, FL |
| 1A District 12 | Higher Seed Host, FL |
| 1A District 13 | FL |
| 1A District 14 | Higher Seed Host, FL |
| 1A District 15 | Higher Seed Host, FL |
| 1A District 16 | Higher Seed Host, FL |
