| 7A District 1 | Mandarin High School , FL |
| 7A District 2 | FL |
| 7A District 3 | Timber Creek , FL |
| 7A District 4 | Lake Brantley , FL |
| 7A District 5 | FL |
| 7A District 6 | Osceola High School , FL |
| 7A District 7 | Higher Seed Hosts, FL |
| 7A District 8 | FL |
| 7A District 9 | Vero Beach , FL |
| 7A District 10 | FL |
| 7A District 11 | Park Vista , FL |
| 7A District 12 | FL |
| 7A District 13 | FL |
| 7A District 14 | Flanagan High School , FL |
| 7A District 15 | Southwest Miami, FL |
| 7A District 16 | Coral Reef , FL |
| 6A District 1 | Navarre, FL |
| 6A District 2 | Higher Seed Hosts, FL |
| 6A District 3 | Fleming Island, FL |
| 6A District 4 | Winter Springs, FL |
| 6A District 5 | FL |
| 6A District 6 | St. Cloud High School, FL |
| 6A District 7 | FL |
| 6A District 8 | FL |
| 6A District 9 | East Lake High School , FL |
| 6A District 10 | FL |
| 6A District 11 | FL |
| 6A District 12 | Higher Seed Hosts, FL |
| 6A District 13 | Martin County High School , FL |
| 6A District 14 | St. Thomas Aquinas , FL |
| 6A District 15 | FL |
| 6A District 16 | Doral Academy , FL |
| 5A District 1 | Gulf Breeze High School , FL |
| 5A District 2 | Choctaw High School , FL |
| 5A District 3 | FL |
| 5A District 4 | FL |
| 5A District 5 | Vanguard High School , FL |
| 5A District 6 | FL |
| 5A District 7 | Jesuit, FL |
| 5A District 8 | FL |
| 5A District 9 | Northeast High School , FL |
| 5A District 10 | FL |
| 5A District 11 | FL |
| 5A District 12 | Higher Seed Hosts, FL |
| 5A District 13 | Eau Gallie, FL |
| 5A District 14 | Okeechobee , FL |
| 5A District 15 | Archbishop McCarthy , FL |
| 5A District 16 | Miami Southridge, FL |
| 4A District 1 | Higher Seed Hosts, FL |
| 4A District 2 | Higher Seed Hosts, FL |
| 4A District 3 | Higher Seed Hosts, FL |
| 4A District 4 | FL |
| 4A District 5 | Top Seed Hosts, FL |
| 4A District 6 | FL |
| 4A District 7 | FL |
| 4A District 8 | Rockledge , FL |
| 4A District 9 | Anclote High School , FL |
| 4A District 10 | Higher Seed Hosts, FL |
| 4A District 11 | Higher Seed Hosts, FL |
| 4A District 12 | Higher Seed Hosts, FL |
| 4A District 13 | FL |
| 4A District 14 | American Heritage School , FL |
| 4A District 15 | Miami Northwestern, FL |
| 4A District 16 | Miami Springs , FL |
| 3A District 1 | Rutherford, FL |
| 3A District 2 | Florida High , FL |
| 3A District 3 | Bishop Snyder , FL |
| 3A District 4 | FL |
| 3A District 5 | Higher Seed Hosts, FL |
| 3A District 6 | Higher Seed Hosts, FL |
| 3A District 7 | Tampa Prep, FL |
| 3A District 8 | Berkeley Prep , FL |
| 3A District 9 | Lawnwood Complex Fort Pierce, FL |
| 3A District 10 | Higher Seed Hosts, FL |
| 3A District 11 | Higher Seed Hosts, FL |
| 3A District 12 | Higher Seed Hosts, FL |
| 3A District 13 | Calvary Christian (Fort Lauderdale), FL |
| 3A District 14 | Monsignor Pace , FL |
| 3A District 15 | Higher Seed Hosts, FL |
| 3A District 16 | Keys Gate, FL |
| 2A District 1 | North Florida Christian , FL |
| 2A District 2 | St. Francis Catholic, FL |
| 2A District 3 | St. Johns Country Day, FL |
| 2A District 4 | St. Josephs (St. Augustine), FL |
| 2A District 5 | Mount Dora Christian Academy, FL |
| 2A District 6 | Orangewood Christian , FL |
| 2A District 7 | Mitchell Ellington Park , FL |
| 2A District 8 | Santa Fe Catholic , FL |
| 2A District 9 | Bishop McLaughlin , FL |
| 2A District 10 | FL |
| 2A District 11 | Higher Seed Hosts, FL |
| 2A District 12 | Satellite sites, FL |
| 2A District 13 | Higher Seed Hosts, FL |
| 2A District 14 | Santaluces Sports Complex, FL |
| 2A District 15 | Westminster Academy, FL |
| 2A District 16 | Miami Christian, FL |
| 1A District 1 | Jay High School , FL |
| 1A District 2 | FL |
| 1A District 3 | Chipley , FL |
| 1A District 4 | Port St. Joe, FL |
| 1A District 5 | Liberty County High School , FL |
| 1A District 6 | Union County High School , FL |
| 1A District 7 | Newberry , FL |
| 1A District 8 | Higher Seed Hosts, FL |
