| 2A District 1 | First Round Higher Seed; Bartram Trail HS, FL |
| 2A District 2 | East Ridge High School, FL |
| 2A District 3 | Lake Brantley High School, FL |
| 2A District 4 | Hagerty High School, FL |
| 2A District 5 | Winter Park High School, FL |
| 2A District 6 | Boone H.S., FL |
| 2A District 7 | Higher Seed Host, FL |
| 2A District 8 | First Round Higher Seed; Melbourne HS, FL |
| 2A District 9 | Wiregrass Ranch HS, FL |
| 2A District 10 | Sickles HS, FL |
| 2A District 11 | Higher Seed Host, FL |
| 2A District 12 | Higher Seed Host, FL |
| 2A District 13 | FL |
| 2A District 14 | Park Vista High School, FL |
| 2A District 15 | South Plantation HS, FL |
| 2A District 16 | Flanagan H.S., FL |
| 1A District 1 | West Florida High School, FL |
| 1A District 2 | South Walton High School, FL |
| 1A District 3 | St. Augustine High School, FL |
| 1A District 4 | Higher Seed Host, FL |
| 1A District 5 | Higher Seed Host, FL |
| 1A District 6 | Bishop Moore HS, FL |
| 1A District 7 | Cocoa Beach Jr./Sr. High School, FL |
| 1A District 8 | Holy Trinity, FL |
| 1A District 9 | Berkeley Preparatory, FL |
| 1A District 10 | FL |
| 1A District 11 | Higher Seed Host, FL |
| 1A District 12 | Higher Seed Host, FL |
| 1A District 13 | Higher Seed Host, FL |
| 1A District 14 | Higher Seed Host, FL |
| 1A District 15 | Calvary Christian Academy, FL |
| 1A District 16 | Higher Seed Host, FL |
