| 1st | Meghan Mulligan | Cherry Creek (Greenwood Village, CO) | Final | | 00:26.56 |
| 2nd | Elana Fisher | Cherry Creek (Greenwood Village, CO) | Final | | 00:28.19 |
| 3rd | Cailey Bosman | Cherry Creek (Greenwood Village, CO) | Final | | 00:28.32 |
| 4th | Pearl Schwartz | Cherry Creek (Greenwood Village, CO) | Final | | 00:28.38 |
| 5th | Lauren Hitt | Cherry Creek (Greenwood Village, CO) | Final | | 00:28.70 |
| 6th | Natalie Weber | Arapahoe (Centennial, CO) | Final | | 00:28.85 |
| 7th | Jordan Barr | Cherry Creek (Greenwood Village, CO) | Final | | 00:29.10 |
| 8th | Sariya Vafina | Cherry Creek (Greenwood Village, CO) | Final | | 00:29.25 |
| 9th | Grace Hollingsworth | Arapahoe (Centennial, CO) | Final | | 00:29.53 |
| 10th | Elsa Jerde | Cherry Creek (Greenwood Village, CO) | Final | | 00:29.69 |
| 11th | Megan Venette | Cherry Creek (Greenwood Village, CO) | Final | | 00:29.71 |
| 12th | Rachel Searle | Arapahoe (Centennial, CO) | Final | | 00:29.98 |
| 13th | Ellen Broady | Arapahoe (Centennial, CO) | Final | | 00:30.25 |
| 14th | Colleen Richards | Arapahoe (Centennial, CO) | Final | | 00:30.26 |
| 15th | Katherine Goodall | Cherry Creek (Greenwood Village, CO) | Final | | 00:30.28 |
| 16th | Lindsey Benkelman | Cherry Creek (Greenwood Village, CO) | Final | | 00:30.28 |
| 17th | Nia Hammond | Cherry Creek (Greenwood Village, CO) | Final | | 00:30.29 |
| 18th | Isabelle Sumner | Arapahoe (Centennial, CO) | Final | | 00:30.29 |
| 19th | Monica Splain | Arapahoe (Centennial, CO) | Final | | 00:30.34 |
| 20th | Leeann Harris | Cherry Creek (Greenwood Village, CO) | Final | | 00:30.59 |
| 21st | Alexa Blessinger | Cherry Creek (Greenwood Village, CO) | Final | | 00:30.61 |
| 22nd | Grace Kercher | Cherry Creek (Greenwood Village, CO) | Final | | 00:30.72 |
| 23rd | Jessica Gouin | Arapahoe (Centennial, CO) | Final | | 00:30.74 |
| 24th | Julia Zykan | Cherry Creek (Greenwood Village, CO) | Final | | 00:30.78 |
| 25th | Sophia Luyten | Cherry Creek (Greenwood Village, CO) | Final | | 00:31.06 |
| 26th | Isabel Tittle | Cherry Creek (Greenwood Village, CO) | Final | | 00:31.22 |
| 27th | Alexandra Crowder | Arapahoe (Centennial, CO) | Final | | 00:31.43 |
| 28th | Lauren Dock | Cherry Creek (Greenwood Village, CO) | Final | | 00:31.43 |
| 29th | Erin Tornello | Arapahoe (Centennial, CO) | Final | | 00:31.56 |
| 30th | Madeline Phillips | Arapahoe (Centennial, CO) | Final | | 00:31.63 |
| 31st | Ciara Coughlan | Arapahoe (Centennial, CO) | Final | | 00:31.72 |
| 32nd | McKenna Lafferty | Cherry Creek (Greenwood Village, CO) | Final | | 00:31.90 |
| 33rd | Bailey Thompson | Arapahoe (Centennial, CO) | Final | | 00:31.96 |
| 34th | Fiona Fruitman | Cherry Creek (Greenwood Village, CO) | Final | | 00:32.00 |
| 35th | Delaney Dyer | Cherry Creek (Greenwood Village, CO) | Final | | 00:32.16 |
| 36th | Allison Bull | Arapahoe (Centennial, CO) | Final | | 00:32.41 |
| 37th | Carli Mastin | Cherry Creek (Greenwood Village, CO) | Final | | 00:32.57 |
| 38th | Kim Connelly | Cherry Creek (Greenwood Village, CO) | Final | | 00:32.62 |
| 39th | Brianna Barsick | Arapahoe (Centennial, CO) | Final | | 00:32.64 |
| 40th | Maddy McCullor | Cherry Creek (Greenwood Village, CO) | Final | | 00:33.19 |
| 41st | Katie Buckley | Arapahoe (Centennial, CO) | Final | | 00:33.48 |
| 42nd | Zoe Zerr | Arapahoe (Centennial, CO) | Final | | 00:33.49 |
| 43rd | Lily Penaloza | Cherry Creek (Greenwood Village, CO) | Final | | 00:33.62 |
| 44th | Reba Mehaffey | Cherry Creek (Greenwood Village, CO) | Final | | 00:33.90 |
| 45th | Peytin Kenney | Cherry Creek (Greenwood Village, CO) | Final | | 00:33.92 |
| 46th | Sarah Porter | Cherry Creek (Greenwood Village, CO) | Final | | 00:33.93 |
| 47th | Renea Michael | Cherry Creek (Greenwood Village, CO) | Final | | 00:33.96 |
| 48th | Ciara Langdon | Cherry Creek (Greenwood Village, CO) | Final | | 00:34.04 |
| 49th | Maddy Tice | Arapahoe (Centennial, CO) | Final | | 00:34.12 |
| 50th | Abigail Jones | Arapahoe (Centennial, CO) | Final | | 00:34.13 |
| 51st | Joey Murray | Cherry Creek (Greenwood Village, CO) | Final | | 00:34.17 |
| 52nd | Allie Highsmith | Arapahoe (Centennial, CO) | Final | | 00:34.79 |
| 53rd | Camryn Caldwell | Cherry Creek (Greenwood Village, CO) | Final | | 00:34.88 |
| 54th | Katelynn Vaccaro | Arapahoe (Centennial, CO) | Final | | 00:35.85 |
| 55th | Jessica Leadbetter | Arapahoe (Centennial, CO) | Final | | 00:36.20 |
| 56th | Esther Gardner | Arapahoe (Centennial, CO) | Final | | 00:37.17 |
| 57th | Ashton Phelps | Arapahoe (Centennial, CO) | Final | | 00:39.08 |
| 58th | Morgan Jenney | Cherry Creek (Greenwood Village, CO) | Final | | 00:39.19 |
| 59th | Nicole Belin | Arapahoe (Centennial, CO) | Final | | 00:39.24 |
| 60th | Reagan Anema | Arapahoe (Centennial, CO) | Final | | 00:40.16 |
| 61st | Andrea Arias | Cherry Creek (Greenwood Village, CO) | Final | | 00:40.82 |
