| 1st | Harris Galvin | Arapahoe (Centennial, CO) | Final | | 00:26.61 |
| 2nd | Kennedy Philbrick | Rock Canyon (Highlands Ranch, CO) | Final | | 00:26.82 |
| 3rd | Anna Case | Rock Canyon (Highlands Ranch, CO) | Final | | 00:27.39 |
| 4th | Hanna Roeser | Rock Canyon (Highlands Ranch, CO) | Final | | 00:27.64 |
| 5th | Jaida Graham | Grandview (Aurora, CO) | Final | | 00:27.68 |
| 6th | Jessica Wang | Rock Canyon (Highlands Ranch, CO) | Final | | 00:27.83 |
| 7th | Julianna Bauer | Rock Canyon (Highlands Ranch, CO) | Final | | 00:27.85 |
| 8th | Mia Finlay | Grandview (Aurora, CO) | Final | | 00:27.93 |
| 9th | Katie Cranston | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.05 |
| 10th | Darby Jester | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.13 |
| 11th | Olivia Luhnau | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.27 |
| 12th | Nia Mantle | Arapahoe (Centennial, CO) | Final | | 00:28.49 |
| 13th | Hannah Stephenson | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.52 |
| 14th | Kendall Babbs | Arapahoe (Centennial, CO) | Final | | 00:28.56 |
| 15th | Samantha Yoder | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.63 |
| 16th | Grace Haas | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.80 |
| 17th | Madeline Alley | Rock Canyon (Highlands Ranch, CO) | Final | | 00:28.83 |
| 18th | Paige Dailey | Grandview (Aurora, CO) | Final | | 00:29.19 |
| 19th | Zella Schnitzpahn | Rock Canyon (Highlands Ranch, CO) | Final | | 00:29.40 |
| 20th | Olivia Learned | Grandview (Aurora, CO) | Final | | 00:29.67 |
| 21st | Mandy Brockamp | Grandview (Aurora, CO) | Final | | 00:29.69 |
| 22nd | Abigail Chalmers | Arapahoe (Centennial, CO) | Final | | 00:29.72 |
| 23rd | Alexandra Lambert | Arapahoe (Centennial, CO) | Final | | 00:29.83 |
| 24th | Teagan Osoba | Rock Canyon (Highlands Ranch, CO) | Final | | 00:29.85 |
| 25th | Meredith Mounier | Grandview (Aurora, CO) | Final | | 00:29.96 |
| 26th | Nicole Cubley | Grandview (Aurora, CO) | Final | | 00:30.10 |
| 27th | Savannah Carr | Arapahoe (Centennial, CO) | Final | | 00:30.14 |
| 28th | Darien Rhoads | Arapahoe (Centennial, CO) | Final | | 00:30.17 |
| 29th | Lasata Ranjitkar | Grandview (Aurora, CO) | Final | | 00:30.20 |
| 30th | Caroline Pita | Rock Canyon (Highlands Ranch, CO) | Final | | 00:30.22 |
| 31st | Aipei Winger | Arapahoe (Centennial, CO) | Final | | 00:30.23 |
| 32nd | Emma Gardner | Arapahoe (Centennial, CO) | Final | | 00:30.25 |
| 33rd | Zoe Wargin | Rock Canyon (Highlands Ranch, CO) | Final | | 00:30.43 |
| 34th | Avery Hicks | Arapahoe (Centennial, CO) | Final | | 00:30.53 |
| 35th | Elaine Musslewhite | Arapahoe (Centennial, CO) | Final | | 00:30.57 |
| 36th | Sophia Stagner | Arapahoe (Centennial, CO) | Final | | 00:30.66 |
| 37th | Susan Kate James | Rock Canyon (Highlands Ranch, CO) | Final | | 00:30.71 |
| 38th | Lydia Dallapiazza | Arapahoe (Centennial, CO) | Final | | 00:30.73 |
| 39th | Mackenna Holmes | Arapahoe (Centennial, CO) | Final | | 00:30.97 |
| 40th | Julia Searle | Arapahoe (Centennial, CO) | Final | | 00:30.97 |
| 41st | Katie Fleming | Rock Canyon (Highlands Ranch, CO) | Final | | 00:30.99 |
| 42nd | Ashley Glader | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.08 |
| 43rd | Amelia Henning | Grandview (Aurora, CO) | Final | | 00:31.25 |
| 44th | Devan Formenti | Arapahoe (Centennial, CO) | Final | | 00:31.26 |
| 45th | Taya Hastings | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.29 |
| 46th | Maggie Wolf | Arapahoe (Centennial, CO) | Final | | 00:31.34 |
| 47th | Amanda Burge | Grandview (Aurora, CO) | Final | | 00:31.35 |
| 48th | Kaeley Elevich | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.42 |
| 49th | Amber Wennerstrom | Arapahoe (Centennial, CO) | Final | | 00:31.62 |
| 50th | Mackenzie Philbrick | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.72 |
| 51st | Camden Krumholz | Arapahoe (Centennial, CO) | Final | | 00:31.80 |
| 52nd | Abby Rickman | Rock Canyon (Highlands Ranch, CO) | Final | | 00:31.82 |
| 53rd | Kaitlyn Powell | Arapahoe (Centennial, CO) | Final | | 00:32.07 |
| 54th | Kenzie Dinkel | Arapahoe (Centennial, CO) | Final | | 00:32.12 |
| 55th | Piper Barnes | Arapahoe (Centennial, CO) | Final | | 00:32.45 |
| 56th | Anna Visscher | Arapahoe (Centennial, CO) | Final | | 00:32.51 |
| 57th | Logan Howe | Arapahoe (Centennial, CO) | Final | | 00:32.59 |
| 58th | Anna Fleming | Rock Canyon (Highlands Ranch, CO) | Final | | 00:32.62 |
| 59th | Sara Robles | Grandview (Aurora, CO) | Final | | 00:32.62 |
| 60th | Arley Stark | Arapahoe (Centennial, CO) | Final | | 00:32.63 |
| 61st | Shreya Rane | Grandview (Aurora, CO) | Final | | 00:32.69 |
| 62nd | Sofia Zalinger | Arapahoe (Centennial, CO) | Final | | 00:33.10 |
| 63rd | Abigail Johnson | Arapahoe (Centennial, CO) | Final | | 00:33.25 |
| 64th | Kimmy Eggen | Arapahoe (Centennial, CO) | Final | | 00:33.25 |
| 65th | Ava Amaral | Arapahoe (Centennial, CO) | Final | | 00:33.27 |
| 66th | Sophia Lesser | Grandview (Aurora, CO) | Final | | 00:33.27 |
| 67th | Kaitlyn Caviness | Arapahoe (Centennial, CO) | Final | | 00:33.29 |
| 68th | Maiken Wat | Arapahoe (Centennial, CO) | Final | | 00:33.37 |
| 69th | Anna Benevento | Rock Canyon (Highlands Ranch, CO) | Final | | 00:33.43 |
| 70th | Darya Mirzakhanova | Grandview (Aurora, CO) | Final | | 00:33.55 |
| 71st | Sierra Jensen | Rock Canyon (Highlands Ranch, CO) | Final | | 00:33.61 |
| 72nd | Abby James | Rock Canyon (Highlands Ranch, CO) | Final | | 00:33.65 |
| 73rd | Brinnli Stevens | Grandview (Aurora, CO) | Final | | 00:33.85 |
| 74th | Lindsey Scott | Rock Canyon (Highlands Ranch, CO) | Final | | 00:33.88 |
| 75th | Lindsay Klaess | Arapahoe (Centennial, CO) | Final | | 00:34.12 |
| 76th | Kylie Waltman | Grandview (Aurora, CO) | Final | | 00:34.57 |
| 77th | Avery Acua | Arapahoe (Centennial, CO) | Final | | 00:34.63 |
| 78th | Abby Mendus | Rock Canyon (Highlands Ranch, CO) | Final | | 00:35.02 |
| 79th | Eva Searle | Arapahoe (Centennial, CO) | Final | | 00:35.11 |
| 80th | Taylor MacArthur | Rock Canyon (Highlands Ranch, CO) | Final | | 00:35.19 |
| 81st | Eva Bianco | Rock Canyon (Highlands Ranch, CO) | Final | | 00:35.19 |
| 82nd | Marx Ferron | Rock Canyon (Highlands Ranch, CO) | Final | | 00:35.23 |
| 83rd | Kaitlin Snodgrass | Arapahoe (Centennial, CO) | Final | | 00:35.35 |
| 84th | Allison Blimmel | Grandview (Aurora, CO) | Final | | 00:35.37 |
| 85th | Lily Kroger | Grandview (Aurora, CO) | Final | | 00:35.65 |
| 86th | Adeline Goujon | Rock Canyon (Highlands Ranch, CO) | Final | | 00:36.13 |
| 87th | Haleigh Bates | Grandview (Aurora, CO) | Final | | 00:36.29 |
| 88th | Harriet Callahan | Grandview (Aurora, CO) | Final | | 00:36.89 |
| 89th | Ella Cooper - Leavitt | Rock Canyon (Highlands Ranch, CO) | Final | | 00:37.21 |
| 90th | Sarah Blair | Grandview (Aurora, CO) | Final | | 00:37.39 |
| 91st | Dakota McCoumb | Arapahoe (Centennial, CO) | Final | | 00:37.45 |
| 92nd | Madeline Ramsey | Rock Canyon (Highlands Ranch, CO) | Final | | 00:37.99 |
| 93rd | Mia Koy | Rock Canyon (Highlands Ranch, CO) | Final | | 00:38.32 |
| 94th | Athena Wright | Arapahoe (Centennial, CO) | Final | | 00:38.65 |
| 95th | Victoria Zazueta | Grandview (Aurora, CO) | Final | | 00:38.74 |
| 96th | Eva Nikov | Grandview (Aurora, CO) | Final | | 00:39.04 |
| 97th | Mia Johnson | Rock Canyon (Highlands Ranch, CO) | Final | | 00:40.56 |
| 98th | Jada Martinez | Grandview (Aurora, CO) | Final | | 00:44.41 |
| 99th | Samantha Blair | Grandview (Aurora, CO) | Final | | 00:45.67 |
